Cloned (Comment) | Organism |
---|---|
wild-type and D10A mutant | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D10A | retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | higher polymerase activity with Mg2+ than with Mn2+, exonuclease acitivity shows equal efficiency with Mg2+ and Mn2+ | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99000 | - |
SDS-PAGE | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Streptococcus pneumoniae | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.84 | - |
- |
Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Streptococcus pneumoniae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity, pol I | Streptococcus pneumoniae | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptococcus pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 55 | highest activity at 37°C, enzyme is also active at 55°C | Streptococcus pneumoniae |