Cloned (Comment) | Organism |
---|---|
polymerase delta, overproduced in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aphidicolin | 50% inhibition at 0.0006 mM | Saccharomyces cerevisiae | |
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate | 50% inhibition at 0.08 mg/ml | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | increases enzymatic activity, no absolute dependence on zinc | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
124000 | - |
polymerase delta, calculated from nucleotide sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Saccharomyces cerevisiae | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
DNA polymerase delta overproduced in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Renatured (Comment) | Organism |
---|---|
renatured by dialysis against decreasing concentrations of urea, optimal protein concentration for refolding: 0.005 mg/ml | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Saccharomyces cerevisiae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity | Saccharomyces cerevisiae | diphosphate + DNAn+1 | - |
? |