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Literature summary for 2.7.7.7 extracted from

  • Brown, W.C.; Duncan, J.A.; Campbell, J.L.
    Purification and characterization of the Saccharomyces cerevisiae DNA polymerase delta overproduced in Escherichia coli (1993), J. Biol. Chem., 268, 982-990.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
polymerase delta, overproduced in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
aphidicolin 50% inhibition at 0.0006 mM Saccharomyces cerevisiae
N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate 50% inhibition at 0.08 mg/ml Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ increases enzymatic activity, no absolute dependence on zinc Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
124000
-
polymerase delta, calculated from nucleotide sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharomyces cerevisiae
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
DNA polymerase delta overproduced in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
renatured by dialysis against decreasing concentrations of urea, optimal protein concentration for refolding: 0.005 mg/ml Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Saccharomyces cerevisiae diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity Saccharomyces cerevisiae diphosphate + DNAn+1
-
?