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Literature summary for 2.7.7.7 extracted from

  • Gass, K.B.; Cozzarelli, N.R.
    Bacillus subtilis DNA polymerases (1974), Methods Enzymol., 29, 27-38.
    View publication on PubMed

General Stability

General Stability Organism
unstable in solutions with low ionic strength, glycerol and bovine serum albumin stabilize Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoic acid
-
Bacillus subtilis
Ara-CTP polymerase II and III Bacillus subtilis
hydroxyphenylazouracil inhibits only polymerase III Bacillus subtilis
KCl
-
Bacillus subtilis
additional information no inhibition of polymerase I by ara-CTP Bacillus subtilis
N-ethylmaleimide polymerase I and II; polymerase III Bacillus subtilis
single-stranded DNA
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Bacillus subtilis polymerase I plays a role in repair of chromosomal damage diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Bacillus subtilis polymerase III is necessary for DNA replication diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polymerase I, II and III Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.042
-
polymerase III Bacillus subtilis
0.4
-
polymerase II Bacillus subtilis

Storage Stability

Storage Stability Organism
-60°C, stable for 60 days Bacillus subtilis
0°C, 3 weeks stable in 0.5 M potassium phosphate, 2 M ammonium sulfate or 4 M NaCl Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn template specificity polymerase I, II and III Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn polymerase I plays a role in repair of chromosomal damage Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn polymerase III is necessary for DNA replication Bacillus subtilis diphosphate + DNAn+1
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
heat stability of polymerase I, II and III Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
hydroxyphenylazouracil pH 7.5, 37°C Bacillus subtilis