Activating Compound | Comment | Organism | Structure |
---|---|---|---|
proliferating cell nuclear antigen | PCNA, specific auxiliary factor stimulating DNA polymerase delta | Drosophila melanogaster | |
proliferating cell nuclear antigen | PCNA, specific auxiliary factor stimulating DNA polymerase delta | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | DNA polymerase alpha: free Mg2+ competes with primer for enzyme binding, dramatic inhibition at Mg2+ concentration above the optimum | Drosophila melanogaster | |
Mg2+ | DNA polymerase alpha: free Mg2+ competes with primer for enzyme binding, dramatic inhibition at Mg2+ concentration above the optimum | Homo sapiens | |
single-stranded DNA | inhibition of polymerase alpha, competitive with respect to activated DNA substrate | Drosophila melanogaster | |
single-stranded DNA | inhibition of polymerase alpha, competitive with respect to activated DNA substrate | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | DNA polymerase alpha: increasing concentrations of Mg2+ lead to a dramatically increased affinity for poly(dT) and poly(dC) polypyrimidines, has little or no effect on the interaction of the enzyme with poly(dA) | Drosophila melanogaster | |
Mg2+ | DNA polymerase alpha: increasing concentrations of Mg2+ lead to a dramatically increased affinity for poly(dT) and poly(dC) polypyrimidines, has little or no effect on the interaction of the enzyme with poly(dA) | Homo sapiens | |
Mg2+ | free Mg2+ competes with primer for enzyme binding, dramatic inhibition at Mg2+ concentration above the optimum, catalytic core binds primer through a Mg2+-chelate, with each of 4 Mg2+ ions acting to coordinate 2 phosphodiester groups | Drosophila melanogaster | |
Mg2+ | free Mg2+ competes with primer for enzyme binding, dramatic inhibition at Mg2+ concentration above the optimum, catalytic core binds primer through a Mg2+-chelate, with each of 4 Mg2+ ions acting to coordinate 2 phosphodiester groups | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Drosophila melanogaster | DNA polymerase delta: with its auxiliary factor i.e. proliferating cell nuclear antigen, largely responsible for leading-strand synthesis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Homo sapiens | DNA polymerase delta: with its auxiliary factor i.e. proliferating cell nuclear antigen, largely responsible for leading-strand synthesis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Drosophila melanogaster | DNA polymerase alpha: with its associated primase largely responsible for lagging-strand synthesis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Homo sapiens | DNA polymerase alpha: with its associated primase largely responsible for lagging-strand synthesis | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Drosophila melanogaster | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Homo sapiens | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | overview: basic mechanism of replicative DNA polymerases alpha and delta | Drosophila melanogaster | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | overview: basic mechanism of replicative DNA polymerases alpha and delta | Homo sapiens | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism of polymerase translocation along templates | Drosophila melanogaster | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism of polymerase translocation along templates | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
KB cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | interaction of polymerases with template-primers containing chemically modified or damaged bases | Drosophila melanogaster | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | interaction of polymerases with template-primers containing chemically modified or damaged bases | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | fidelity of DNA replication | Drosophila melanogaster | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | fidelity of DNA replication | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase delta: with its auxiliary factor i.e. proliferating cell nuclear antigen, largely responsible for leading-strand synthesis | Drosophila melanogaster | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase delta: with its auxiliary factor i.e. proliferating cell nuclear antigen, largely responsible for leading-strand synthesis | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase alpha: with its associated primase largely responsible for lagging-strand synthesis | Drosophila melanogaster | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | DNA polymerase alpha: with its associated primase largely responsible for lagging-strand synthesis | Homo sapiens | diphosphate + DNAn+1 | - |
? |