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Literature summary for 2.7.7.60 extracted from

  • Tsang, A.; Seidle, H.; Jawaid, S.; Zhou, W.; Smith, C.; Couch, R.
    Francisella tularensis 2-C-Methyl-D-Erythritol 4-phosphate Cytidylyltransferase: Kinetic characterization and phosphoregulation (2011), PLoS ONE, 6, e20884.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Francisella tularensis

Protein Variants

Protein Variants Comment Organism
T141D mutant shows only 25% of wild-type activity Francisella tularensis
T141E mutant catalytically inactive Francisella tularensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
 CTP pH 8.0, 37°C Francisella tularensis
0.178
-
 2-C-methyl-D-erythritol 4-phosphate pH 8.0, 37°C Francisella tularensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ preferred cation Francisella tularensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25900
-
 predicted from cDNA Francisella tularensis

Organism

Organism UniProt Comment Textmining
Francisella tularensis
-
-
-
Francisella tularensis FSC155
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Francisella tularensis

Purification (Commentary)

Purification (Comment) Organism
using metal affinity chromatography Francisella tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Francisella tularensis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Francisella tularensis FSC155 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration Francisella tularensis

Synonyms

Synonyms Comment Organism
MEP cytidylyltransferase
-
 Francisella tularensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Francisella tularensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 CTP pH 8.0, 37°C Francisella tularensis
1
-
 2-C-methyl-D-erythritol 4-phosphate pH 8.0, 37°C Francisella tularensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Francisella tularensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.66
-
 2-C-methyl-D-erythritol 4-phosphate pH 8.0, 37°C Francisella tularensis