Application | Comment | Organism |
---|---|---|
drug development | due to the absence of the pathway in mammals enzyme provides a potential target for new antibiotics | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli M15 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
CTP | pH 7.5, 30°C | Escherichia coli | |
0.061 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 7.5, 30°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2-C-methyl-D-erythritol 4-phosphate | Escherichia coli | essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2-C-methyl-D-erythritol 4-phosphate | - |
Escherichia coli | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol 4-phosphate | essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis | Escherichia coli | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Escherichia coli |