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Literature summary for 2.7.7.6 extracted from

  • Palm, P.; Schleper, C.; Arnold-Ammer, I.; Holz, I.; Meier, T.; Lottspeich, F.; Zillig, W.
    The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits (1993), Nucleic Acids Res., 21, 4904-4908.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the genes for the large subunits beta and beta‘ are cloned and sequenced. MW for beta‘ and beta, calculated from nucleotide sequence is 190522 Da and 143139 Da Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima
55000
-
beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima
141000
-
beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima
184000
-
beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn the enzyme requires DNA as template Thermotoga maritima diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn the enzyme is highly active with poly dAT or T7 phage DNA as template Thermotoga maritima diphosphate + RNAn+1
-
?

Subunits

Subunits Comment Organism
? beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima