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Literature summary for 2.7.7.6 extracted from

  • Patra, D.; Lafer, E.M.; Sousa, R.
    Isolation and characterization of mutant bacteriophage T7 RNA polymerases (1992), J. Mol. Biol., 224, 307-318.
    View publication on PubMed
Search for more literature for 2.7.7.6

Protein Variants

Protein Variants Comment Organism
additional information RNAP mutants: the N-terminal region of T7 RNAP contains a nascent RNA binding site that functions to retain the nascent chain within the ternary complex. The region surrounding residue 240 is involved in binding the initiating NTP. Residues at the very C terminus of T7 RNAP are involved in binding the elongating NTP Escherichia phage T7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.079 0.107 UTP wild-type enzyme Escherichia phage T7
0.14 0.142 ATP wild-type enzyme Escherichia phage T7
0.143 0.18 CTP wild-type enzyme Escherichia phage T7
0.234
-
 GTP wild-type enzyme Escherichia phage T7

Organism

Organism UniProt Comment Textmining
Escherichia phage T7
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNAn
-
 Escherichia phage T7 diphosphate + RNAn+1
-
 ?
CTP + RNAn
-
 Escherichia phage T7 diphosphate + RNAn+1
-
 ?
GTP + RNAn
-
 Escherichia phage T7 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn the enzyme requires DNA as template Escherichia phage T7 diphosphate + RNAn+1
-
 ?
UTP + RNAn
-
 Escherichia phage T7 diphosphate + RNAn+1
-
 ?