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Literature summary for 2.7.7.6 extracted from
- Cyanobacterial DNA-dependent RNA polymerase (1988), Methods Enzymol., 167, 592-599.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| enzyme shows an extreme instability in the presence of salts | Microchaete diplosiphon |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, maximal activity at 10-20 mM | Synechococcus elongatus PCC 7942 = FACHB-805 |  |
| Mg2+ | required, maximal activity at 10-20 mM | Anabaena sp. | |
| Mg2+ | only limited ability to replace Mn2+ | Anabaena cylindrica | |
| Mg2+ | 30 mM Mg2+ or 5 mM Mn2+ work equally well for maximal activity | Microchaete diplosiphon | |
| Mn2+ | can partially replace Mg2+ in activation | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Mn2+ | can partially replace Mg2+ in activation | Anabaena sp. | |
| Mn2+ | 30 mM Mg2+ or 5 mM Mn2+ work equally well for maximal activity | Microchaete diplosiphon | |
| Mn2+ | 2-4 mM required for optimal activity | Anabaena cylindrica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
| 38000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
| 41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
| 41000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
| 41000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
| 47000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
| 52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
| 52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
| 52000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
| 66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
| 66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
| 66000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
| 72000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
| 72000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 91000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
| 93000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
| 124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
| 124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
| 124000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
| 125000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
| 134000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
| 145000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 161000 | - |
x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
| 171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
| 171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
| 171000 | - |
x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
| 185000 | - |
x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
| 190000 | - |
x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena cylindrica | - |
- |
- |
| Anabaena sp. | - |
PCC 7120 | - |
| Microchaete diplosiphon | - |
- |
- |
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PCC 7120 | Anabaena sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0156 | - |
- |
Anabaena sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Synechococcus elongatus PCC 7942 = FACHB-805 | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Anabaena sp. | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Anabaena cylindrica | diphosphate + RNAn+1 | - |
? | |
| nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Microchaete diplosiphon | diphosphate + RNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| ? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena sp. |
| ? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Anabaena cylindrica |
| ? | x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE | Microchaete diplosiphon |
| ? | x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE | Microchaete diplosiphon |
| ? | x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
| ? | x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE | Anabaena cylindrica |
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