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Literature summary for 2.7.7.56 extracted from

  • Jain, C.
    Novel role for RNase PH in the degradation of structured RNA (2012), J. Bacteriol., 194, 3883-3890.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.56

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNAn+1 + phosphate Escherichia coli the enzyme is a 3' to 5' RNase. The enzyme digests through RNA duplexes of moderate stability tRNAn + a nucleoside diphosphate
-
 ?
tRNAn+1 + phosphate Escherichia coli MG1655 the enzyme is a 3' to 5' RNase. The enzyme digests through RNA duplexes of moderate stability tRNAn + a nucleoside diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MG1655
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNAn+1 + phosphate the enzyme is a 3' to 5' RNase. The enzyme digests through RNA duplexes of moderate stability Escherichia coli tRNAn + a nucleoside diphosphate
-
 ?
tRNAn+1 + phosphate the enzyme is a 3' to 5' RNase. RNase PH uses phosphate as a nucleophile to catalyze RNA cleavage Escherichia coli tRNAn + a nucleoside diphosphate
-
 ?
tRNAn+1 + phosphate the enzyme is a 3' to 5' RNase. The enzyme digests through RNA duplexes of moderate stability Escherichia coli MG1655 tRNAn + a nucleoside diphosphate
-
 ?
tRNAn+1 + phosphate the enzyme is a 3' to 5' RNase. RNase PH uses phosphate as a nucleophile to catalyze RNA cleavage Escherichia coli MG1655 tRNAn + a nucleoside diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
RNase PH
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction absence of RNase PH and polynucleotide phosphorylase, EC 2.7.7.8, causes growth defects. Absence of both enzymes results in the appearance and accumulation of novel mRNA degradation fragments, which are also observed in strains containing mutations in RNase R and PNPase, enzymes whose collective absence is known to cause an accumulation of structured RNA fragments. Single or double deletion of either pnp or rph had a moderate effect on the rpsO, trxA, or lpp mRNAs Escherichia coli
physiological function role for RNase PH in the degradation of structured RNA, overview Escherichia coli