Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method, wild-type and triple mutant R68Q-R73Q-R76Q RNase PH crystallized and the structures determined by X-ray diffraction to medium resolution. Wild-type and triple mutant RNase PH crystallize as a hexamer and dimer, respectively | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
R68Q-R73Q-R76Q | triple mutant RNase PH crystallize as a dimer, compared to wild-type enzyme that crystallizes as a hexamer | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAn+1 + phosphate | Bacillus subtilis | involved in the maturation of tRNA precursors and especially important for removal of nucleotide residues near the CCA acceptor end of the mature tRNAs | tRNAn + a nucleoside diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
recombinant | - |
Purification (Comment) | Organism |
---|---|
recombinant | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAn+1 + phosphate | - |
Bacillus subtilis | tRNAn + a nucleoside diphosphate | - |
? | |
tRNAn+1 + phosphate | involved in the maturation of tRNA precursors and especially important for removal of nucleotide residues near the CCA acceptor end of the mature tRNAs | Bacillus subtilis | tRNAn + a nucleoside diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase PH | - |
Bacillus subtilis |