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Literature summary for 2.7.7.56 extracted from

  • Harlow, L.S.; Kadziola, A.; Jensen, K.F.; Larsen, S.
    Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding (2004), Protein Sci., 13, 668-677.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method, wild-type and triple mutant R68Q-R73Q-R76Q RNase PH crystallized and the structures determined by X-ray diffraction to medium resolution. Wild-type and triple mutant RNase PH crystallize as a hexamer and dimer, respectively Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
R68Q-R73Q-R76Q triple mutant RNase PH crystallize as a dimer, compared to wild-type enzyme that crystallizes as a hexamer Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNAn+1 + phosphate Bacillus subtilis involved in the maturation of tRNA precursors and especially important for removal of nucleotide residues near the CCA acceptor end of the mature tRNAs tRNAn + a nucleoside diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
recombinant
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNAn+1 + phosphate
-
Bacillus subtilis tRNAn + a nucleoside diphosphate
-
?
tRNAn+1 + phosphate involved in the maturation of tRNA precursors and especially important for removal of nucleotide residues near the CCA acceptor end of the mature tRNAs Bacillus subtilis tRNAn + a nucleoside diphosphate
-
?

Synonyms

Synonyms Comment Organism
RNase PH
-
Bacillus subtilis