Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
tRNA-C-C-A-C2 | pH 8.0, 37°C | Escherichia coli | |
0.001 | - |
tRNA-C-C-A-C3 | pH 8.0, 37°C | Escherichia coli | |
2 | - |
phosphate | with tRNA-C-C-A-C2 or tRNA-C-C-A-C3, pH 8.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 30% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM | Escherichia coli | |
KCl | 50-75 mM stimulates 2fold | Escherichia coli | |
Mg2+ | maximal at 5-10 mM | Escherichia coli | |
Mn2+ | 40% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM | Escherichia coli | |
additional information | no effect: Zn2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | 50000 | gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNAn+1 + phosphate | Escherichia coli | role in tRNA processing and RNA degradation | tRNA + a nucleoside diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene rph, orfE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + phosphate | - |
Escherichia coli | ? | - |
r | |
tRNA-C-C-A-C2 + phosphate | - |
Escherichia coli | ? | - |
? | |
tRNA-C-C-A-C3 + phosphate | - |
Escherichia coli | ? | - |
? | |
tRNAn+1 + phosphate | substrate specificity | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
tRNAn+1 + phosphate | dependent on phosphate | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
tRNAn+1 + phosphate | role in tRNA processing and RNA degradation | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, fairly stable up to | Escherichia coli |
55 | - |
10 min, about 50% loss of activity | Escherichia coli |
65 | - |
10 min, complete inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Escherichia coli |