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Literature summary for 2.7.7.56 extracted from

  • Kelly, K.O.; Deutscher, M.P.
    Characterization of Escherichia coli RNase PH (1992), J. Biol. Chem., 267, 17153-17158.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
tRNA-C-C-A-C2 pH 8.0, 37°C Escherichia coli
0.001
-
tRNA-C-C-A-C3 pH 8.0, 37°C Escherichia coli
2
-
phosphate with tRNA-C-C-A-C2 or tRNA-C-C-A-C3, pH 8.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 30% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM Escherichia coli
KCl 50-75 mM stimulates 2fold Escherichia coli
Mg2+ maximal at 5-10 mM Escherichia coli
Mn2+ 40% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM Escherichia coli
additional information no effect: Zn2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000 50000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNAn+1 + phosphate Escherichia coli role in tRNA processing and RNA degradation tRNA + a nucleoside diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene rph, orfE
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + phosphate
-
Escherichia coli ?
-
r
tRNA-C-C-A-C2 + phosphate
-
Escherichia coli ?
-
?
tRNA-C-C-A-C3 + phosphate
-
Escherichia coli ?
-
?
tRNAn+1 + phosphate substrate specificity Escherichia coli tRNA + a nucleoside diphosphate
-
r
tRNAn+1 + phosphate dependent on phosphate Escherichia coli tRNA + a nucleoside diphosphate
-
r
tRNAn+1 + phosphate role in tRNA processing and RNA degradation Escherichia coli tRNA + a nucleoside diphosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, fairly stable up to Escherichia coli
55
-
10 min, about 50% loss of activity Escherichia coli
65
-
10 min, complete inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Escherichia coli