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Literature summary for 2.7.7.52 extracted from

  • Tu, B.; Liu, L.; Xu, C.; Zhai, J.; Li, S.; Lopez, M.A.; Zhao, Y.; Yu, Y.; Ramachandran, V.; Ren, G.; Yu, B.; Li, S.; Meyers, B.C.; Mo, B.; Chen, X.
    Distinct and cooperative activities of HESO1 and URT1 nucleotidyl transferases in microRNA turnover in Arabidopsis (2015), PLoS Genet., 11, e1005119.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.52

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O64642
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Arabidopsis thaliana Q5XET5
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information HESO1 exhibits nucleotidyl transferase activity on methylated miRNA in vitro. HESO1 has a clear preference for miR158A-U. miR158A-G is the second most preferred substrate Arabidopsis thaliana ?
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 ?
additional information URT1 exhibits nucleotidyl transferase activity on unmethylated miRNA in vitro. URT1 shows a strong preference for miR158 ending in A, miR158A-C, miR158A-G, and miR158A-U are similarly used by URT1 Arabidopsis thaliana ?
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 ?

Synonyms

Synonyms Comment Organism
HESO1
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 Arabidopsis thaliana
URT1
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 Arabidopsis thaliana
UTP:RNA uridylyltransferase 1
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 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function isoform HESO1, which uridylates most unmethylated miRNAs in vivo, and isoform URT1 which exhibits nucleotidyl transferase activity on unmethylated miRNA, prefer substrates with different 3'-end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Both HESO1 and URT1 exhibit nucleotidyl transferase activity on AGO1-bound miRNAs. Although the enzymes are able to add long tails to AGO1-bound miRNAs, the tailed miRNAs remain associated with AGO1. Tailing of AGO1-bound miRNA165/6 drastically reduces the slicing activity of AGO1-miR165/6 Arabidopsis thaliana
physiological function URT1 is the single most predominant nucleotidyl transferase that tails miRNAs. URT1 and isoform HESO1, which uridylates most unmethylated miRNAs in vivo, prefer substrates with different 3'-end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Both HESO1 and URT1 exhibit nucleotidyl transferase activity on AGO1-bound miRNAs. Although the enzymes are able to add long tails to AGO1-bound miRNAs, the tailed miRNAs remain associated with AGO1. Tailing of AGO1-bound miRNA165/6 drastically reduces the slicing activity of AGO1-miR165/6. Monouridylation of miR171a by URT1 endows the miRNA the ability to trigger the biogenesis of secondary siRNAs Arabidopsis thaliana