Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein TbMP81 | editosome protein, interaction enhances the enzyme activity | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
DNA sequence determination, editosomal enzyme, expression as His-tagged protein in Escherichia coli | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Trypanosoma brucei | 5739 | - |
additional information | integral editosome protein, distinct from mitochondrial enzyme form | Trypanosoma brucei | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
mitochondrial enzyme form, gel filtration | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + RNAn | Trypanosoma brucei | addition of primarily single U to single-stranded RNA, addition of the number of Us specified by a guide RNA to insertion editing-like substrates | diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
editosome protein TbMP57, distinct from mitochondrial enzyme form, i.e. TbTUTase 108 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNAn | editosomal enzyme form, no activity | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
CTP + RNAn | editosomal enzyme form, no activity | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
GTP + RNAn | editosomal enzyme form, no activity | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | editosomal enzyme form shows preference for a 3' terminal A or G, while the mitochondrial enzyme form does not | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | mitochondrial enzyme form adds Us at the 3' and the 5' end of the RNA | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | addition of primarily single U to single-stranded RNA, addition of the number of Us specified by a guide RNA to insertion editing-like substrates | Trypanosoma brucei | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3' terminal uridylyl transferase | - |
Trypanosoma brucei |