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Literature summary for 2.7.7.50 extracted from
- Enzymatic synthesis of RNAs capped with nucleotide analogues reveals the molecular basis for substrate selectivity of RNA capping enzyme: impacts on RNA metabolism (2013), PLoS ONE, 8, e75310.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-fluoro-2'-deoxy-GTP | - |
Paramecium bursaria Chlorella virus 1 |  |
| 2-amino-6-chloropurine-ribose triphosphate | - |
Paramecium bursaria Chlorella virus 1 | |
| 3'-deoxy-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| 3'-O-Methyl-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| 6-methylthio-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| 6-thio-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| 8-bromo-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| 8-iodoGTP | - |
Paramecium bursaria Chlorella virus 1 | |
| GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| ITP | - |
Paramecium bursaria Chlorella virus 1 | |
| N1-methyl-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
| O6-methyl-GTP | - |
Paramecium bursaria Chlorella virus 1 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paramecium bursaria Chlorella virus 1 | Q84424 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-fluoro-2'-deoxy-GTP + pp(5')RNA | 0.16fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 2'-fluoro-2'-deoxy-G(5')ppp(5')RNA + diphosphate | - |
? | |
| 2-amino-6-chloropurine-beta-D-ribose triphosphate + pp(5')RNA | 0.08fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 2-amino-6-chloropurine-beta-D-ribose-(5')ppp(5')RNA + diphosphate | - |
? | |
| 3'-deoxy-GTP + pp(5')RNA | 0.15fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 3'-deoxy-G(5')ppp(5')RNA + diphosphate | - |
? | |
| 3'-O-methyl-GTP + pp(5')RNA | 0.38fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 3'-O-methyl-G(5')ppp(5')RNA + diphosphate | - |
? | |
| 6-methylthio-GTP + pp(5')RNA | 0.07fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 6-methylthio-G(5')ppp(5')RNA + diphosphate | - |
? | |
| 6-thio-GTP + pp(5')RNA | 0.16fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 6-thio-G(5')ppp(5')RNA + diphosphate | - |
? | |
| 8-bromo-GTP + pp(5')RNA | 0.40fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 8-bromo-G(5')ppp(5')RNA + diphosphate | - |
? | |
| 8-iodoGTP + pp(5')RNA | 1.0fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | 8-iodo-G(5')ppp(5')RNA + diphosphate | - |
? | |
| GTP + pp(5')RNA | - |
Paramecium bursaria Chlorella virus 1 | G(5')ppp(5')RNA + diphosphate | - |
? | |
| ITP + pp(5')RNA | 1.3fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | I(5')ppp(5')RNA + diphosphate | - |
? | |
| additional information | generation of RNAs harboring various different cap analogues. Cap-dependent translation can occur in the absence of theN7-methyl group on the cap structure provided that alternative modifications enable appropriate binding to eIF4E | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
| N1-methyl-GTP + pp(5')RNA | 1.1fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | N1-methyl-G(5')ppp(5')RNA + diphosphate | - |
? | |
| O6-methyl-GTP + pp(5')RNA | 0.48fold efficiency compared with substrate GTP | Paramecium bursaria Chlorella virus 1 | O6-methyl-G(5')ppp(5')RNA + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| A103R | - |
Paramecium bursaria Chlorella virus 1 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | N1-methyl-GTP | |
| 0.1 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | GTP | |
| 0.15 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | O6-methyl-GTP | |
| 0.15 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 3'-deoxy-GTP | |
| 0.16 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 2-amino-6-chloropurine-ribose triphosphate | |
| 0.22 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 6-thio-GTP | |
| 0.34 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | ITP | |
| 0.42 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 8-iodoGTP | |
| 0.43 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 6-methylthio-GTP | |
| 0.45 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 3'-O-Methyl-GTP | |
| 1.4 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 2'-fluoro-2'-deoxy-GTP | |
| 1.5 | - |
IC50 value for formation of enzyme-GMP-complex, pH 7.5, 30°C | Paramecium bursaria Chlorella virus 1 | 8-bromo-GTP | |
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