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Literature summary for 2.7.7.50 extracted from

  • Bullard-Feibelman, K.M.; Fuller, B.P.; Geiss, B.J.
    A sensitive and robust high-throughput screening assay for inhibitors of the Chikungunya virus nsP1 capping enzyme (2016), PLoS ONE, 11, e0158923.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.50

Application

Application Comment Organism
analysis fluorescence polarization-based assay that monitors displacement of a fluorescently-labeled GTP analog in real time and a secondary orthogonal assay that measures guanylation activity. This platform provides an avenue for identification of potent nsP1 inhibitors Chikungunya virus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chikungunya virus

Inhibitors

Inhibitors Comment Organism Structure
7-methyl-GTP
-
 Chikungunya virus
ATP
-
 Chikungunya virus
ddGTP
-
 Chikungunya virus
GpppA
-
 Chikungunya virus
GTP
-
 Chikungunya virus
ITP
-
 Chikungunya virus
XTP
-
 Chikungunya virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
-
 Chikungunya virus

Organism

Organism UniProt Comment Textmining
Chikungunya virus Q1H8W7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-[(6-amino)hexyl]-amino-GTP-ATTO-680 + pp(5')RNA fluorescent substrate analog Chikungunya virus G(5')ppp(5')RNA + diphosphate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE, x * 61088, mass spectrometry, recombinant protein with His-tag Chikungunya virus

Synonyms

Synonyms Comment Organism
nsP1
-
 Chikungunya virus