Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda cells via infection with baculovirus | bluetongue virus serotype 10 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
bluetongue virus serotype 10 | - |
mino core protein VP4 | - |
bluetongue virus serotype 10 | - |
from infected Spodoptera frugiperda cells via baculovirus | - |
bluetongue virus serotype 10 | - |
i.e. BTV serotype 10 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Spodoptera frugiperda cells after infection with baculovirus, to homogeneity | bluetongue virus serotype 10 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')GpXp | - |
bluetongue virus serotype 10 | G(5')ppp(5')GpXp + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | reversible formation of a covalent intermediate enzyme-GMP | bluetongue virus serotype 10 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
GTP + pp(5')RNA | GMP is linked to the enzyme via a phosphoamide bond | bluetongue virus serotype 10 | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
additional information | enzyme also catalyzes GTP-diphosphate exchange | bluetongue virus serotype 10 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the capping enzyme complex contains RNA 5'-triphosphatase, RNA guanylyltransferase and pyrophosphatase activities | bluetongue virus serotype 10 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | bluetongue virus serotype 10 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | bluetongue virus serotype 10 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme-GMP complex is resistant to alkali, but is cleaved at acidic conditions with HCl or NH2OH | bluetongue virus serotype 10 |