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Literature summary for 2.7.7.50 extracted from
- Purification and characterization of the messenger ribonucleic acid capping enzyme GTP:RNA guanylyltransferase from wheat germ (1982), Biochemistry, 21, 327-333.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | 50% at 0.005 mM, complete at 0.05 mM | Triticum aestivum |  |
| phosphate | weak | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000014 | - |
pp(5')A(pA)n | pH 7.9, 37°C | Triticum aestivum | |
| 0.0027 | - |
GTP | pH 7.9, 37°C | Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | can partially replace Mn2+ in activation | Triticum aestivum | |
| Mn2+ | required | Triticum aestivum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2000fold | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for more than 6 months, considerably longer at -70°C | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + pp(5')A(pA)n | - |
Triticum aestivum | ? | - |
? | |
| GTP + pp(5')RNA | - |
Triticum aestivum | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
| additional information | specificity overview | Triticum aestivum | ? | - |
? | |
| additional information | little sequence specificity for RNA acceptor | Triticum aestivum | ? | - |
? | |
| additional information | no acceptor: RNA with a single 5'-terminal phosphate | Triticum aestivum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Triticum aestivum |
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Release 2023.1 (January 2023)
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