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Literature summary for 2.7.7.49 extracted from

  • Hartl, M.J.; Mayr, F.; Rethwilm, A.; Woehrl, B.M.
    Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases (2010), Retrovirology, 7, 5-15.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged protease domain in Escherichia coli strain BL21 (DE3) pREP4:GroESL Simian foamy virus

Inhibitors

Inhibitors Comment Organism Structure
azidothymidine inhibits foamy virus replication prototype foamy virus
azidothymidine inhibits FV replication Simian foamy virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0401
-
dTTP determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain prototype foamy virus
0.045
-
deoxynucleoside triphosphate determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain prototype foamy virus
0.045
-
dTTP determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain Simian foamy virus
0.046
-
deoxynucleoside triphosphate determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain Simian foamy virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Simian foamy virus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn prototype foamy virus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
prototype foamy virus
-
gene pol
-
Simian foamy virus
-
gene pol
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protease domain from Escherichia coli strain BL21 (DE3) pREP4:GroESL by nickel affinity and hydrophobic interaction chromatography Simian foamy virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Simian foamy virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
prototype foamy virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn heteropolymeric single stranded M13 substrate Simian foamy virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn heteropolymeric single stranded M13 substrate prototype foamy virus diphosphate + DNAn+1
-
?
dTTP + DNAn homopolymeric substrate poly(rA)/oligo(dT) Simian foamy virus diphosphate + DNAn+1
-
?
dTTP + DNAn homopolymeric substrate poly(rA)/oligo(dT) prototype foamy virus diphosphate + DNAn+1
-
?
additional information the N-terminal protease domain of the reverse transcriptase also shows polymerase activity Simian foamy virus ?
-
?

Subunits

Subunits Comment Organism
dimer hydrophobic interactions might promote dimerization Simian foamy virus
dimer hydrophobic interactions might promote dimerization prototype foamy virus
More the FV reverse transcriptase harbors a protease, polymerase and RNase H domain Simian foamy virus

Synonyms

Synonyms Comment Organism
FV Pol
-
Simian foamy virus
FV Pol
-
prototype foamy virus
FV reverse transcriptase
-
Simian foamy virus
PFV RT
-
prototype foamy virus
prototype foamy virus reverse transcriptase
-
prototype foamy virus
SFV RT
-
Simian foamy virus
simian foamy virus reverse transcriptase
-
Simian foamy virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
deoxynucleoside triphosphate determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain Simian foamy virus
4
-
deoxynucleoside triphosphate determined for dNTPs on a heteropolymeric single stranded M13 substrate, pH and temperature not specified in the publication, recombinant His-tagged protease domain prototype foamy virus
5.5
-
dTTP determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain prototype foamy virus
7.1
-
dTTP determined for TTP on the homopolymeric substrate poly(rA)/oligo(dT), pH and temperature not specified in the publication, recombinant His-tagged protease domain Simian foamy virus