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Literature summary for 2.7.7.49 extracted from

  • Schoenbrunner, N.J.; Fiss, E.H.; Budker, O.; Stoffel, S.; Sigua, C.L.; Gelfand, D.H.; Myers, T.W.
    Chimeric thermostable DNA polymerases with reverse transcriptase and attenuated 3-5 exonuclease activity (2006), Biochemistry, 45, 12786-12795.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol and containing the 5'-3' nuclease domain from Thermus sp. Z05 DNA polymerase (residues 1-291) and the 3'-5' exonuclease and polymerase domains from Thermotoga maritima DNA polymerase (residues 292-893). This chimera retains thermostable DNA polymerase activity, as well as proofreading activity. Using the CS5 chimera, a series of mutant proteins is constructed in which the amino acid side chains are mutated to modulate the 3'-5' exonuclease activity. The chimeric DNA polymerases are overexpressed under the control of the lambda PL promoter are expressed in Escherichia coli Thermus sp.
chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol and containing the 5'-3' nuclease domain from Thermus sp. Z05 DNA polymerase (residues 1-291) and the 3'-5' exonuclease and polymerase domains from Thermotoga maritima DNA polymerase (residues 292-893). This chimera retains thermostable DNA polymerase activity, as well as proofreading activity. Using the CS5 chimera, a series of mutant proteins is constructed in which the amino acid side chains are mutated to modulate the 3'-5' exonuclease activity. The chimeric DNA polymerases are overexpressed under the control of the lambda PL promoter are expressed in Escherichia coli Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
additional information chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol and containing the 5'-3' nuclease domain from Thermus sp. Z05 DNA polymerase (residues 1-291) and the 3'-5' exonuclease and polymerase domains from Thermotoga maritima DNA polymerase (residues 292-893). This chimera retains thermostable DNA polymerase activity, as well as proofreading activity. Using the CS5 chimera, a series of mutant proteins is constructed in which the amino acid side chains are mutated to modulate the 3'-5'?exonuclease activity. A thermoactive and thermostable enzyme with reverse transcriptase and 3'-5'exonuclease activity is described Thermotoga maritima
additional information chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol and containing the 5'-3' nuclease domain from Thermussp. Z05 DNA polymerase (residues 1-291) and the 3'-5' exonuclease and polymerase domains from Thermotoga maritima DNA polymerase (residues 292-893). This chimera retains thermostable DNA polymerase activity, as well as proofreading activity. Using the CS5 chimera, a series of mutant proteins is constructed in which the amino acid side chains are mutated to modulate the 3'-5' exonuclease activity. A thermoactive and thermostable enzyme with reverse transcriptase and 3'-5'?exonuclease activity is described Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermus sp.
-
-
-
Thermus sp. Z05
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus sp.
-
 Thermotoga maritima

Synonyms

Synonyms Comment Organism
CS5 pol chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol Thermus sp.
CS5 pol chimeric DNA polymerase, termed CS5 pol, constructed from T. Z05 pol and Tma pol Thermotoga maritima
T. Z05 pol
-
 Thermus sp.
T. Z05 pol
-
 Thermotoga maritima