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Literature summary for 2.7.7.49 extracted from

  • Cases-Gonzalez, C.E.; Menendez-Arias, L.
    Nucleotide specificity of HIV-1 reverse transcriptases with amino acid substitutions affecting Ala-114 (2005), Biochem. J., 387, 221-229.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
A114G mutant enzyme retains significant DNA polymerase activity. 5-6fold increase in the ability of the enzyme to discriminate against ddNTPs Human immunodeficiency virus 1
A114S mutant enzyme retains significant DNA polymerase activity, enzyme shows wild-type ddNTP/dNTP discrimination efficiency Human immunodeficiency virus 1
A114T mutant enzyme shows very low catalytic efficiency in nucleotide incorporation assays, due to the high KM-values for dNTP. Decreased susceptibility to AZTTP when poly(rA)/(dT)16 is used as substrate Human immunodeficiency virus 1
A114V mutant enzyme shows very low catalytic efficiency in nucleotide incorporation assays, due to the high KM-values for dNTP. Decreased susceptibility to AZTTP when poly(rA)/(dT)16 is used as substrate, enzyme shows wild-type ddNTP/dNTP discrimination efficiency Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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 additional information
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 Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
-
-

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
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 additional information
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 Human immunodeficiency virus 1