Application | Comment | Organism |
---|---|---|
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Avian myeloblastosis virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Rous sarcoma virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | murine leukemia virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Mason-Pfizer monkey virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Reticuloendotheliosis virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | mouse mammary tumor virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Woolly monkey sarcoma virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Hamster leukemia virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Gibbon ape leukemia virus |
synthesis | reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling | Squirrel monkey retrovirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
1 * 53000 + 1 * 68000, SDS-PAGE | Hamster leukemia virus |
68000 | - |
- |
Gibbon ape leukemia virus |
68000 | - |
1 * 53000 + 1 * 68000, SDS-PAGE | Hamster leukemia virus |
70000 | - |
- |
Woolly monkey sarcoma virus |
70000 | 84000 | - |
Reticuloendotheliosis virus |
80000 | 100000 | - |
Mason-Pfizer monkey virus |
80000 | 100000 | - |
Squirrel monkey retrovirus |
100000 | - |
- |
mouse mammary tumor virus |
120000 | - |
- |
Hamster leukemia virus |
170000 | - |
- |
Avian myeloblastosis virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Avian myeloblastosis virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Rous sarcoma virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | murine leukemia virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Mason-Pfizer monkey virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Reticuloendotheliosis virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | mouse mammary tumor virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Woolly monkey sarcoma virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Hamster leukemia virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Gibbon ape leukemia virus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Squirrel monkey retrovirus | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avian myeloblastosis virus | - |
- |
- |
Gibbon ape leukemia virus | - |
- |
- |
Hamster leukemia virus | - |
- |
- |
Mason-Pfizer monkey virus | - |
- |
- |
mouse mammary tumor virus | - |
- |
- |
murine leukemia virus | - |
- |
- |
Reticuloendotheliosis virus | - |
- |
- |
Rous sarcoma virus | - |
- |
- |
Squirrel monkey retrovirus | - |
- |
- |
Woolly monkey sarcoma virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Avian myeloblastosis virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Rous sarcoma virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Mason-Pfizer monkey virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Reticuloendotheliosis virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | mouse mammary tumor virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Woolly monkey sarcoma virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Hamster leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Gibbon ape leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H | Squirrel monkey retrovirus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Avian myeloblastosis virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Rous sarcoma virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Mason-Pfizer monkey virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Reticuloendotheliosis virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | mouse mammary tumor virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Woolly monkey sarcoma virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Hamster leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Gibbon ape leukemia virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections | Squirrel monkey retrovirus | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 53000 + 1 * 68000, SDS-PAGE | Hamster leukemia virus |