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Literature summary for 2.7.7.49 extracted from

  • Verma, I.M.
    Reverse transcriptase (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 87-103.
No PubMed abstract available

Application

Application Comment Organism
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Avian myeloblastosis virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Rous sarcoma virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling murine leukemia virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Mason-Pfizer monkey virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Reticuloendotheliosis virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling mouse mammary tumor virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Woolly monkey sarcoma virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Hamster leukemia virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Gibbon ape leukemia virus
synthesis reverse transcriptase is commonly used to synthesize DNA complementary to a variety of RNA templates, synthesis of cDNA. Reverse transcriptase can utilize single-stranded DNA or RNA-DNA hybrid as template to synthesize double-stranded DNA. The reverse transcriptase, unlike the bacterial DNA polymerase, lacks the 3'-5' and 5'-3' exonuclease and can thus be efficiently used for end labeling or gap filling Squirrel monkey retrovirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
1 * 53000 + 1 * 68000, SDS-PAGE Hamster leukemia virus
68000
-
-
Gibbon ape leukemia virus
68000
-
1 * 53000 + 1 * 68000, SDS-PAGE Hamster leukemia virus
70000
-
-
Woolly monkey sarcoma virus
70000 84000
-
Reticuloendotheliosis virus
80000 100000
-
Mason-Pfizer monkey virus
80000 100000
-
Squirrel monkey retrovirus
100000
-
-
mouse mammary tumor virus
120000
-
-
Hamster leukemia virus
170000
-
-
Avian myeloblastosis virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Avian myeloblastosis virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Rous sarcoma virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn murine leukemia virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Mason-Pfizer monkey virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Reticuloendotheliosis virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn mouse mammary tumor virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Woolly monkey sarcoma virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Hamster leukemia virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Gibbon ape leukemia virus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Squirrel monkey retrovirus enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Avian myeloblastosis virus
-
-
-
Gibbon ape leukemia virus
-
-
-
Hamster leukemia virus
-
-
-
Mason-Pfizer monkey virus
-
-
-
mouse mammary tumor virus
-
-
-
murine leukemia virus
-
-
-
Reticuloendotheliosis virus
-
-
-
Rous sarcoma virus
-
-
-
Squirrel monkey retrovirus
-
-
-
Woolly monkey sarcoma virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Avian myeloblastosis virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Rous sarcoma virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H murine leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Mason-Pfizer monkey virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Reticuloendotheliosis virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H mouse mammary tumor virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Woolly monkey sarcoma virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Hamster leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Gibbon ape leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme exhibits both synthetic and degradative activity, DNA polymerase and RNAse H Squirrel monkey retrovirus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Avian myeloblastosis virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Rous sarcoma virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections murine leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Mason-Pfizer monkey virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Reticuloendotheliosis virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections mouse mammary tumor virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Woolly monkey sarcoma virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Hamster leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Gibbon ape leukemia virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn enzyme plays a central role during the life cycle of a retrovirus. Temperature-sensitive mutants with a lesion in the reverse transcriptase are unable to establish infections Squirrel monkey retrovirus diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
dimer 1 * 53000 + 1 * 68000, SDS-PAGE Hamster leukemia virus