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Literature summary for 2.7.7.49 extracted from

  • Wang, H.; Kennell, J.C.; Kuiper, M.T.R.; Sabourin, J.R.; Saldanha, R.; Lambowitz, A.M.
    The Mauriceville plasmid of Neurospora crassa: characterization of a novel reverse transcriptase that begins cDNA synthesis at the 3 end of template RNA (1992), Mol. Cell. Biol., 12, 5131-5144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Neurospora crassa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Neurospora crassa 5739
-
ribonucleoprotein complex
-
Neurospora crassa 1990904
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) at 300 mM KCl, optimal activity with poly(rC)-oligo(dG)12-18 at 200 mM Neurospora crassa
Mg2+ optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) at1 mM Mg2+, optimal activity with poly(rC)-oligo(dG)12-18 at 5-10 mM Mg2+ Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
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-
-

Purification (Commentary)

Purification (Comment) Organism
partial Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme synthesizes full-length cDNA copies of in vitro transcripts beginning at the 3'-end and has a preference for transcripts having the 3'tRNA-like structure. The enzyme begins cDNA synthesis directly opposite the 3'-terminal nucleotide of the template RNA. The activity with poly(rC) alone is about 5% of that with poly(rC)*oligo(dG), efficient use of the substrate is dependent on the primer Neurospora crassa diphosphate + DNAn+1
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30 optimal activity with poly(rC)-oligo(dG)12-18 Neurospora crassa
30 37 optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) and with poly(rC)-oligo(dG)12-18 Neurospora crassa