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Literature summary for 2.7.7.49 extracted from

  • Kacian, D.L.; Spiegelman, S.
    Purification and detection of reverse transcriptase in viruses and cells (1974), Methods Enzymol., 29E, 150-173.
No PubMed abstract available
Search for more literature for 2.7.7.49

Activating Compound

Activating Compound Comment Organism Structure
additional information sulfhydryl reducing agent is required for maximal activity, optimal concentration is 100 mM Avian myeloblastosis virus

General Stability

General Stability Organism
rapid inactivation by repeated freezing and thawing Avian myeloblastosis virus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cations are not required, but afforded a severalfold stimulation, optimal concentration is 100 mM Avian myeloblastosis virus
Mg2+ absolute requirement for a divalent cation, optimal concentration: 5-9 mM Avian myeloblastosis virus
Mn2+ absolute requirement for a divalent cation, optimal concentration: 0.8 mM Avian myeloblastosis virus

Organism

Organism UniProt Comment Textmining
Avian myeloblastosis virus
-
 BAI strain
-
Avian myeloblastosis virus BAI
-
 BAI strain
-

Purification (Commentary)

Purification (Comment) Organism
-
 Avian myeloblastosis virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Avian myeloblastosis virus

Storage Stability

Storage Stability Organism
-20°C, 50% v/v glycerol, purified enzyme retains activity for about 6 months, enzyme also retains activity when stored frozen at -70°C Avian myeloblastosis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme accepts a variety of natural RNA templates, but shows a preference for oncogenic virus RNA. RNA from other oncogenic viruses is as efficient as AMV RNA. Homopolymeric duplexes are exceptionally good templates, stimulating synthesis 100fold greater than natural RNA or DNA. The enzyme requires a primer Avian myeloblastosis virus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn the enzyme accepts a variety of natural RNA templates, but shows a preference for oncogenic virus RNA. RNA from other oncogenic viruses is as efficient as AMV RNA. Homopolymeric duplexes are exceptionally good templates, stimulating synthesis 100fold greater than natural RNA or DNA. The enzyme requires a primer Avian myeloblastosis virus BAI diphosphate + DNAn+1
-
 ?

Subunits

Subunits Comment Organism
? two polypeptide chains are detected by SDS-PAGE: 69000 Da and 110000 Da Avian myeloblastosis virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.5
-
 Avian myeloblastosis virus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 activity falls off rapidly below pH 7.5 and much less rapidly above pH 8.5 Avian myeloblastosis virus