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Literature summary for 2.7.7.49 extracted from
- RNA tumor virus DNA polymerases (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 211-235.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-(2,4-dinitrophenylhydrazonomethyl) rifamycin SV | - |
murine leukemia virus |  |
| 3-cyclic amine derivative of rifamycin SV | 0.2 mg/ml, more than 90% inhibition | Harvey murine sarcoma virus | |
| 3-piperazinoiminomethyl rifamycin SV | - |
murine leukemia virus | |
| N-methylisatin beta-thiosemicarbazone | 0.4 mM, 88% inhibition with a 70S RSV RNA template-primer and 50% inhibition with a calf thymus DNA template-primer in the presence of 1% 2-mercaptoethanol | Rous sarcoma virus | |
| NEM | 2 mM, 82% loss of activity | Rauscher murine leukemia virus | |
| p-mercuribenzoate | 0.02 mM, 96% loss of activity | Rauscher murine leukemia virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
dATP | reaction with poly(dA-dT) | Rauscher murine leukemia virus | |
| 0.026 | - |
dTTP | reaction with poly(dA-dT) | Rauscher murine leukemia virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Rous sarcoma virus | |
| K+ | 5 mM, 50% stimulation of activity with poly(dA-dT) | Rauscher murine leukemia virus | |
| K+ | 100 mM KCl, 2fold stimulation | Avian myeloblastosis virus | |
| Mg2+ | - |
Rous sarcoma virus | |
| Mg2+ | - |
murine leukemia virus | |
| Mg2+ | Km: 2.5 mM, reaction with poly(dA-dT) | Rauscher murine leukemia virus | |
| Mg2+ | optimal Mg2+ concentration is 10 mM, reaction with AMV RNA | Avian myeloblastosis virus | |
| Mn2+ | - |
Rous sarcoma virus | |
| Mn2+ | - |
murine leukemia virus | |
| Mn2+ | - |
Rauscher murine leukemia virus | |
| Mn2+ | Mg2+ can partially be replaced by 1 mM Mn2+ | Avian myeloblastosis virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme exists in two forms with different sizes: 65000 Da and 105000 Da | Avian myeloblastosis virus |
| 70000 | - |
gel filtration | Rauscher murine leukemia virus |
| 90000 | - |
velocity centrifugation in a glycerol gradient containing 0.05 M KCl | Rauscher murine leukemia virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avian myeloblastosis virus | - |
- |
- |
| Harvey murine sarcoma virus | - |
- |
- |
| murine leukemia virus | - |
- |
- |
| Rauscher murine leukemia virus | - |
- |
- |
| Rous sarcoma virus | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, 50-70% loss of activity after 1 month | Rauscher murine leukemia virus |
| -20°C, less than 10% loss of activity after 5 weeks | Avian myeloblastosis virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dATP + DNAn | - |
Rauscher murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | - |
Harvey murine sarcoma virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | enzyme requires a 3'-OH group on a primer and carrying out synthesis from the 5' to the 3' end of the molecule, that is by addition of nucleoside monophosphates at the 3'-OH end of the primer. Poly(rA) is almost totally inactive as a template until a primer, either poly(dT) or oligo(dT) is added | Avian myeloblastosis virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | enzyme requires a 3'-OH group on a primer and carrying out synthesis from the 5' to the 3' end of the molecule, that is by addition of nucleoside monophosphates at the 3'-OH end of the primer. Poly(rA) is almost totally inactive as a template until a primer, either poly(dT) or oligo(dT) is added | Rous sarcoma virus | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | enzyme requires a 3'-OH group on a primer and carrying out synthesis from the 5' to the 3' end of the molecule, that is by addition of nucleoside monophosphates at the 3'-OH end of the primer. Poly(rA) is almost totally inactive as a template until a primer, either poly(dT) or oligo(dT) is added | Rauscher murine leukemia virus | diphosphate + DNAn+1 | - |
? | |
| dTTP + poly(rA)/(dT)18 | - |
Rauscher murine leukemia virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | two polypeptides are detected by SDS-PAGE: 70000 Da and 110000 Da | Avian myeloblastosis virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
reaction with RSV RNA, poly(rA)*poly(dT) and DNA | Rous sarcoma virus |
| 8.2 | - |
reaction with AMV RNA | Avian myeloblastosis virus |
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