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Literature summary for 2.7.7.49 extracted from

  • Inouye, S.; Inouye, M.
    Bacterial reverse transcriptase (1993), Cold Spring Harbor Monogr. Ser., 23, 391-410.
No PubMed abstract available
Search for more literature for 2.7.7.49

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Escherichia coli
67227
-
 x * 67227, calculation from nucleotide sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Myxococcus xanthus
-
-
-
Stigmatella aurantiaca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
it is important to purify RT-Ec67 from an Escherichia coli strain defective in DNA polymerase I, because this enzyme can utilize an RNA template to synthesize DNA Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Myxococcus xanthus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 Stigmatella aurantiaca diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn the purified enzyme can synthesize DNA using RNA as a template and a synthetic oligodeoxynucleotide as a primer: cDNA can be synthesized using the Escherichi coli 5S RNA as template and a 15-base synthetic oligonucleotide complementary to the 3'-end of the 5S RNA as a primer. The enzyme can also produce a full-length cDNA using a 50-base synthetic DNA as a template and a synthetic oligonucleotide complementary to the 3'-end of the template as a primer Escherichia coli diphosphate + DNAn+1
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Escherichia coli
? x * 67227, calculation from nucleotide sequence Escherichia coli