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Literature summary for 2.7.7.49 extracted from

  • Temin, H.M.; Mizutani, S.
    RNA-dependent DNA polymerase in virions of Rous sarcoma virus (1970), Nature, 226, 1211-1213.
    View publication on PubMed
Search for more literature for 2.7.7.49

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol to observe full activity of the enzyme, it is necessary to treat the virions with a non-ionic detergent. If the treatment is at 40°C the presence of dithiothreitol is necessary to recover activity Rous sarcoma virus
Nonionic detergent to observe full activity of the enzyme, it is necessary to treat the virions with a non-ionic detergent. If the treatment is at 40°C the presence of dithiothreitol is necessary to recover activity Rous sarcoma virus

Inhibitors

Inhibitors Comment Organism Structure
KCl 80 mM, 60% inhibition Rous sarcoma virus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cation is not required for activity, 20 mM KCl causes 15% stimulation Rous sarcoma virus
Mg2+ absolute requirement, optimal concentration: 10 mM Rous sarcoma virus
Mn2+ MnCl2 can substitute for MgCl2 Rous sarcoma virus

Organism

Organism UniProt Comment Textmining
Rous sarcoma virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn all four deoxyribonucleotide triphosphates are required for full activity, some activity is present when only three deoxyribonucleotide triphosphates are added and 10-20% of full activity is still present with only two deoxyribonucleotide triphosphates Rous sarcoma virus diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
 Rous sarcoma virus