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Literature summary for 2.7.7.48 extracted from

  • Jin, Z.; Leveque, V.; Ma, H.; Johnson, K.A.; Klumpp, K.
    Assembly, purification, and pre-steady-state kinetic analysis of active RNA-dependent RNA polymerase elongation complex (2012), J. Biol. Chem., 287, 10674-10683.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.48

Application

Application Comment Organism
analysis assembly of a productive isoform NS5B primer-template elongation complex stalled after formation of a 9-nucleotide primer. Under optimized conditions up to 65% of NS5B can be converted into active elongation complexes. The elongation complex is extremely stable, allowing purification away from excess nucleotide and abortive initiation products Hepacivirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 CTP pH 7.5, 30°C Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C AJ242654
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + RNA9
-
 Hepacivirus C diphosphate + RNA10
-
 ?

Synonyms

Synonyms Comment Organism
NS5B
-
 Hepacivirus C

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 CTP pH 7.5, 30°C Hepacivirus C

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
410
-
 CTP pH 7.5, 30°C Hepacivirus C