Protein Variants | Comment | Organism |
---|---|---|
S282T | the mutation shows reduced sensitivity to 2'-hydroxy-2'-C-methyl nucleosides | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine 5'-triphosphate | PSI-6130-TP, potent inhibitor | Hepacivirus C | |
beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine 5'-triphosphate | also named RO2433-TP or PSI-6206, potent inhibitor | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS5B protein | - |
Hepacivirus C |
RDRP | - |
Hepacivirus C |
RNA-dependent RNA polymerase | - |
Hepacivirus C |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000059 | - |
beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine 5'-triphosphate | wild type enzyme, with 5 mM MgCl2, and 2 mM dithiothreitol in 50 mM HEPES buffer, pH 7.5 | Hepacivirus C | |
0.00022 | - |
beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine 5'-triphosphate | mutant enzyme S282T, with 5 mM MgCl2, and 2 mM dithiothreitol in 50 mM HEPES buffer, pH 7.5 | Hepacivirus C | |
0.00031 | - |
beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine 5'-triphosphate | mutant enzyme S282T, with 5 mM MgCl2, and 2 mM dithiothreitol in 50 mM HEPES buffer, pH 7.5 | Hepacivirus C | |
0.00042 | - |
beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine 5'-triphosphate | wild type enzyme, with 5 mM MgCl2, and 2 mM dithiothreitol in 50 mM HEPES buffer, pH 7.5 | Hepacivirus C |