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Literature summary for 2.7.7.48 extracted from

  • Johnson, R.B.; Sun, X.L.; Hockman, M.A.; Villarreal, E.C.; Wakulchik, M.; Wang, Q.M.
    Specificity and mechanism analysis of Hepatitis C virus RNA-dependent RNA polymerase (2000), Arch. Biochem. Biophys., 377, 129-134.
    View publication on PubMed
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a nonfusion protein in Escherichia coli Hepacivirus C

Protein Variants

Protein Variants Comment Organism
additional information
-
 Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Hepacivirus C
heparin IC50: 80 ng/ml Hepacivirus C
NaCl 150 mM or above, about 90% of acctivity is lost Hepacivirus C
Ni2+
-
 Hepacivirus C
polylysine IC50: 250 ng/ml Hepacivirus C
Zn2+
-
 Hepacivirus C

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, Ca2+ shows about 20% of the activity with Mg2+ at about 1 mM Hepacivirus C
Mg2+ absolute requirement for divalent cation, effective concentration range is 5-10 mM Hepacivirus C
Mn2+ absolute requirement for divalent cation, effective concentration is about 1 mM Hepacivirus C
NaCl 15-40 mM, 2 to 4fold stimulation Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
 expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
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