Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | rhinovirus A16 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the activity of the 6His-tagged enzyme HRV-16 3D polymerase is identical to HRV-16 3D polymerase without the 6His-tag | rhinovirus A16 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | IC50: 0.0053 mM | rhinovirus A16 | |
CTP | IC50: 0.0015 mM | rhinovirus A16 | |
dATP | IC50 above 0.5 mM | rhinovirus A16 | |
dCTP | IC50: 0.04 mM | rhinovirus A16 | |
ddATP | IC50 above 1 mM | rhinovirus A16 | |
ddCTP | IC50 above 1 mM | rhinovirus A16 | |
ddGTP | IC50: above 0.2 mM | rhinovirus A16 | |
ddTTP | IC50 above 0.5 mM | rhinovirus A16 | |
dGTP | IC50: 0.1 mM | rhinovirus A16 | |
dTTP | IC50 above 0.5 mM | rhinovirus A16 | |
gliotoxin | IC50: 0.15 mM | rhinovirus A16 | |
GTP | IC50: 0.0002 mM | rhinovirus A16 | |
K+ | above 10 mM | rhinovirus A16 | |
Mn2+ | polymerase activity drops sharply at 3 mM | rhinovirus A16 | |
additional information | the enzyme is resistant to 0.02 mg/ml rifampicin | rhinovirus A16 | |
UTP | IC50: 0.0023 mM | rhinovirus A16 | |
Zn2+ | IC50: 0.0006 mM, suppression of inhibition by dithiothreitol | rhinovirus A16 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
CTP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.001 | - |
GTP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.0021 | - |
UTP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.007 | - |
poly(C)n | pH 7.0, 30°C, poly(C) as template | rhinovirus A16 | |
0.008 | - |
ATP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.012 | - |
poly(A)n | pH 7.0, 30°C, poly(A) as template | rhinovirus A16 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | modest stimulation below 10 mM | rhinovirus A16 | |
Mg2+ | in presence of Mg2+ significant activity is observed when poly(A) or poly(C) is used as template and the activity is template and primer-dependent. Poly(G) and poly(U) templates are not efficient substrates. Biotinylated oligoDNA primers appear to work slightly more efficiently than oligoRNA primers. Divalent cation required, optimal concentration for Mg2+ is 1 mM | rhinovirus A16 | |
Mn2+ | in presence of Mn2+ activity is stimulates by 2.5-5.6fold. RNA synthesis using poly(C) as template becomes primer-independent, about 2.5fold stimulation at 1 mM | rhinovirus A16 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, SDS-PAGE | rhinovirus A16 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | rhinovirus A16 | required for replication of the HRV RNA genome | diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
rhinovirus A16 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
rhinovirus A16 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sshRNAn | - |
rhinovirus A16 | diphosphate + sshRNAn+1 | - |
? | |
CTP + sshRNAn | - |
rhinovirus A16 | diphosphate + sshRNAn+1 | - |
? | |
GTP + poly(C) | - |
rhinovirus A16 | diphosphate + poly(C)n+1 | - |
? | |
GTP + poly(C)n | - |
rhinovirus A16 | diphosphate + poly(C)n+1 | - |
? | |
GTP + sshRNAn | - |
rhinovirus A16 | diphosphate + sshRNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | in presence of Mg2+ significant activity is observed when poly(A) or poly(C) is used as template and the activity is template and primer-dependent. Poly(G) and poly(U) templates are not efficient substrates. Biotinylated oligoDNA primers appear to work slightly more efficiently than oligoRNA primers. In presence of Mn2+ activity is stimulated 2.5-5.6fold. RNA synthesis using poly(C) as template becomes primer-independent | rhinovirus A16 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | RNA polymerase activity on homopolymeric templates poly(A) and poly(C) and heteropolymeric RNA templates primed with either RNA or DNA oligonucleotide primers or self-primed by a copy-back mechanism | rhinovirus A16 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | required for replication of the HRV RNA genome | rhinovirus A16 | diphosphate + RNAn+1 | - |
? | |
UTP + poly(A)n | - |
rhinovirus A16 | diphosphate + poly(A)n+1 | - |
? | |
UTP + sshRNAn | - |
rhinovirus A16 | diphosphate + sshRNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | rhinovirus A16 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
poly(A) as template and biotinylated oligo(dU)15 as the primer | rhinovirus A16 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
17 | 37 | 17°C: about 20% of maximal activity, dramatic drecrease in activity between 32°C and 37°C, 37°C: no activity, poly(A) as template and biotinylated oligo(dU)15 as the primer | rhinovirus A16 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000505 | - |
CTP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.000617 | - |
ATP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.00375 | - |
GTP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.00478 | - |
UTP | pH 7.0, 30°C, sshRNA as template | rhinovirus A16 | |
0.0211 | - |
GTP | pH 7.0, 30°C, poly(C) as template | rhinovirus A16 | |
0.23 | - |
UTP | pH 7.0, 30°C, poly(A) as template | rhinovirus A16 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
poly(A) as template and biotinylated oligo(dU)15 as the primer | rhinovirus A16 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | pH 6.5: about 50% of maximal activity, pH 9.0: about 55% of maximal activity, poly(A) as template and biotinylated oligo(dU)15 as the primer | rhinovirus A16 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
IC50: 0.0002 mM | rhinovirus A16 | GTP | |
0.0006 | - |
IC50: 0.0006 mM, suppression of inhibition by dithiothreitol | rhinovirus A16 | Zn2+ | |
0.0015 | - |
IC50: 0.0015 mM | rhinovirus A16 | CTP | |
0.0023 | - |
IC50: 0.0023 mM | rhinovirus A16 | UTP | |
0.0053 | - |
IC50: 0.0053 mM | rhinovirus A16 | ATP | |
0.04 | - |
IC50: 0.04 mM | rhinovirus A16 | dCTP | |
0.1 | - |
IC50: 0.1 mM | rhinovirus A16 | dGTP | |
0.15 | - |
IC50: 0.15 mM | rhinovirus A16 | gliotoxin | |
0.2 | - |
IC50: above 0.2 mM | rhinovirus A16 | ddGTP | |
0.5 | - |
IC50 above 0.5 mM | rhinovirus A16 | dATP | |
0.5 | - |
IC50 above 0.5 mM | rhinovirus A16 | dTTP | |
0.5 | - |
IC50 above 0.5 mM | rhinovirus A16 | ddTTP | |
1 | - |
IC50 above 1 mM | rhinovirus A16 | ddCTP | |
1 | - |
IC50 above 1 mM | rhinovirus A16 | ddATP |