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Literature summary for 2.7.7.48 extracted from

  • Tomei, L.; Vitale, R.L.; Incitti, I.; Serafini, S.; Altamura, S.; Vitelli, A.; De Francesco, R.
    Biochemical characterization of a Hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence (2000), J. Gen. Virol., 81, 759-767.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli Hepacivirus C

Protein Variants

Protein Variants Comment Organism
additional information C-terminal hydrophobic domain deletion mutant, NS5B DELTAC21, shows increased solubility, the deletion also positively affects the polymerase activity Hepacivirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00019
-
GTP pH 7.5, 23°C, deletion mutant DELTAC21, poly(C)-oligo(G)18 as template Hepacivirus C
0.00027
-
GTP pH 7.5, 23°C, full-length enzyme, poly(C)-oligo(G)18 as template Hepacivirus C
0.0049
-
UTP pH 7.5, 23°C, full-length enzyme, poly(A)-oligo(U)18 as template Hepacivirus C
0.0054
-
UTP pH 7.5, 23°C, deletion mutant DELTAC21, poly(A)-oligo(U)18 as template Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
full-length enzyme and C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli or in Sf9 cells Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + RNAn
-
Hepacivirus C diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn
-
Hepacivirus C diphosphate + RNAn+1
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
23
-
20 min, preincubation in absence of RNA, up to 90% loss of activity Hepacivirus C
37
-
20 min, preincubation in absence of RNA, complete loss of activity Hepacivirus C

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
GTP pH 7.5, 23°C, full-length enzyme, poly(C)-oligo(G)18 as template Hepacivirus C
0.00108
-
GTP pH 7.5, 23°C, deletion mutant DELTAC21, poly(C)-oligo(G)18 as template Hepacivirus C
0.00373
-
UTP pH 7.5, 23°C, full-length enzyme, poly(A)-oligo(U)18 as template Hepacivirus C
0.00583
-
UTP pH 7.5, 23°C, deletion mutant DELTAC21, poly(A)-oligo(U)18 as template Hepacivirus C