Cloned (Comment) | Organism |
---|---|
C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli | Hepacivirus C |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal hydrophobic domain deletion mutant, NS5B DELTAC21, shows increased solubility, the deletion also positively affects the polymerase activity | Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00019 | - |
GTP | pH 7.5, 23°C, deletion mutant DELTAC21, poly(C)-oligo(G)18 as template | Hepacivirus C | |
0.00027 | - |
GTP | pH 7.5, 23°C, full-length enzyme, poly(C)-oligo(G)18 as template | Hepacivirus C | |
0.0049 | - |
UTP | pH 7.5, 23°C, full-length enzyme, poly(A)-oligo(U)18 as template | Hepacivirus C | |
0.0054 | - |
UTP | pH 7.5, 23°C, deletion mutant DELTAC21, poly(A)-oligo(U)18 as template | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
full-length enzyme and C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli or in Sf9 cells | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
20 min, preincubation in absence of RNA, up to 90% loss of activity | Hepacivirus C |
37 | - |
20 min, preincubation in absence of RNA, complete loss of activity | Hepacivirus C |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
GTP | pH 7.5, 23°C, full-length enzyme, poly(C)-oligo(G)18 as template | Hepacivirus C | |
0.00108 | - |
GTP | pH 7.5, 23°C, deletion mutant DELTAC21, poly(C)-oligo(G)18 as template | Hepacivirus C | |
0.00373 | - |
UTP | pH 7.5, 23°C, full-length enzyme, poly(A)-oligo(U)18 as template | Hepacivirus C | |
0.00583 | - |
UTP | pH 7.5, 23°C, deletion mutant DELTAC21, poly(A)-oligo(U)18 as template | Hepacivirus C |