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Literature summary for 2.7.7.48 extracted from

  • Ikegami, M.; Fraenkel-Conrat, H.
    Characterization of the RNA-dependent RNA polymerase of tobacco leaves (1979), J. Biol. Chem., 254, 149-154.
    View publication on PubMed
Search for more literature for 2.7.7.48

General Stability

General Stability Organism
the purified enzyme loses most of its activity within 45 min when dialyzed against 10 mM Tris-HCl, pH 8.1, 0.025 M NH4Cl, 0.01 M Kcl, containing only 5% glycerol and no mercaptoethanol, but little activity is lost under these conditions if the glycerol concentration is raised to 30% Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
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 tobacco contains an RNA replicating capability, the amount of which is increased by infection with an RNA virus without noticeable changes in its enzymatic properties
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Purification (Commentary)

Purification (Comment) Organism
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 Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
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 Nicotiana tabacum
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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-
 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn the random polymers poly(UG), poly(UC), poly(AG) and poly(AU) serve as more effective templates than homopolymers Nicotiana tabacum diphosphate + RNAn+1
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