Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is lowered at ionic strength of 0.1 | Enterobacteria phage GA | |
RNase | RNAse A, 0.01 mg/ml, or Rnase T1, 0.002 mg/ml, inhibit the reaction by 90-95% | Enterobacteria phage GA |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacteria phage GA | - |
Escherichia coli infected with | - |
Purification (Comment) | Organism |
---|---|
- |
Enterobacteria phage GA |
Storage Stability | Organism |
---|---|
-20°C, 25% glycerol, half-life is 2 months | Enterobacteria phage GA |
0°C, 25% glycerol, half-life is 10 d | Enterobacteria phage GA |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Enterobacteria phage GA | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains four different subunits: I, II, II and IV. The molecular weights of which are 74000 Da, 60000 Da, 47000 Da and 36000 Da, respectively. Three of them, I, III and IV are host-coded proteins, ribosomal protein S1 and elongation factor Tu(III) and Ts(IV) | Enterobacteria phage GA |