Activating Compound | Comment | Organism | Structure |
---|---|---|---|
RNA | 2-10fold stimulation with the addition of various RNA templates. CCMV and CPMV RNA produces the greatest stimulation. TMV RNA also acts as a template, but with much less efficiency | Maize dwarf mosaic virus | |
Sodium diethyldithiocarbamate | 5 mM, great stimulation of template-dependent activity and to a lesser extent template-independent activity | Maize dwarf mosaic virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 12.5 mM, replicase activity is reduced by 50% | Maize dwarf mosaic virus | |
RNase | completely eliminates incorporation | Maize dwarf mosaic virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme is associated with particulate fraction of Zea mays leaf infected with Maize dwarf mosaic virus | Maize dwarf mosaic virus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 2.8 mM | Maize dwarf mosaic virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
glycerol density gradient sedimentation | Maize dwarf mosaic virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Maize dwarf mosaic virus | - |
Zea mays infected with | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | leaf of Zea mays infected with Maize dwarf mosaic virus | Maize dwarf mosaic virus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Maize dwarf mosaic virus | diphosphate + RNAn+1 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Maize dwarf mosaic virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 9.7 | pH 6.3: about 45% of maximal activity, pH 9.7: about 35% of maximal activity | Maize dwarf mosaic virus |