Organic Solvent | Comment | Organism |
---|---|---|
urea | denaturation | Qubevirus durum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Qubevirus durum | - |
Escherichia coli infected with | - |
Qubevirus durum | - |
QbetaamB86 | - |
Qubevirus durum QbetaamB86 | - |
QbetaamB86 | - |
Purification (Comment) | Organism |
---|---|
- |
Qubevirus durum |
Renatured (Comment) | Organism |
---|---|
when the enzyme is denatured in 8 M urea, a maximum of about 40% of the poly(G) polymerase activity can be regained | Qubevirus durum |
Storage Stability | Organism |
---|---|
-20°C, enzyme concentration greater than 1 mg/ml, stable for more than 1 year | Qubevirus durum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | GTP and polyC | Qubevirus durum QbetaamB86 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the enzyme contains four nonidentical polypeptide chains, only one of which is a product of the phage genome. The other three subunits, present in uninfected cells are ribosomal protein S1 and protein synthesis elongation factors EF-Tu and EF-TS | Qubevirus durum |