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Literature summary for 2.7.7.47 extracted from

  • Chen, Y.; Naesvall, J.; Wu, S.; Andersson, D.I.; Selmer, M.
    Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3)(9) adenyltransferase (2015), Acta Crystallogr. Sect. D, 71, 2267-2277.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.5 A resolution. AadA consists of a nucleotidyltransferase domain and an alpha-helical bundle domain. AadA crystallizes as a monomer. ATP binding has to occur before binding of the aminoglycoside substrate, and ATP binding repositions the two domains for aminoglycoside binding in the interdomain cleft Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
D182A mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
D182N mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
E87A mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain Salmonella enterica subsp. enterica serovar Typhimurium
E87Q mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain Salmonella enterica subsp. enterica serovar Typhimurium
K205A mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
R192A mutation reduces the MIC of streptomycin and spectinomycin Salmonella enterica subsp. enterica serovar Typhimurium
W112A mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin Salmonella enterica subsp. enterica serovar Typhimurium
W112F mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q8ZPX9
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + spectinomycin
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Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + 9-adenylylspectinomycin
-
?
ATP + streptomycin
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Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + 3''-adenylylstreptomycin
-
?

Subunits

Subunits Comment Organism
monomer small-angle X-ray scattering Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
aadA
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Salmonella enterica subsp. enterica serovar Typhimurium

pI Value

Organism Comment pI Value Maximum pI Value
Salmonella enterica subsp. enterica serovar Typhimurium calculated
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5