Crystallization (Comment) | Organism |
---|---|
to 2.5 A resolution. AadA consists of a nucleotidyltransferase domain and an alpha-helical bundle domain. AadA crystallizes as a monomer. ATP binding has to occur before binding of the aminoglycoside substrate, and ATP binding repositions the two domains for aminoglycoside binding in the interdomain cleft | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
D182A | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
D182N | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
E87A | mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain | Salmonella enterica subsp. enterica serovar Typhimurium |
E87Q | mutation reduces the MIC of streptomycin and spectinomycin to that of an AadA null strain | Salmonella enterica subsp. enterica serovar Typhimurium |
K205A | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
R192A | mutation reduces the MIC of streptomycin and spectinomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
W112A | mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
W112F | mutation reduces the MIC of spectinomycin to that of an AadA null strain and reduces the MIC of streptomycin | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZPX9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + spectinomycin | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + 9-adenylylspectinomycin | - |
? | |
ATP + streptomycin | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + 3''-adenylylstreptomycin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | small-angle X-ray scattering | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
aadA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | calculated | - |
5 |