Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | required | Artemia salina |
General Stability | Organism |
---|---|
albumin, 10 mg/ml, glycerol, 30% or P1,P4-bis(5'-guanosyl)tetraphosphate, 0.6 mM, prevents loss of activity at 0°C, Gp4G most effective | Artemia salina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Artemia salina | |
additional information | no inhibition by phosphate | Artemia salina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 28% of the activity with Mg2+ in P1,P4-bis(5'-guanosyl)tetraphosphate synthesis | Artemia salina | |
Mg2+ | 20 mM required with GDP as substrate | Artemia salina | |
Mg2+ | 10-15 mM required for maximal activity with GTP as substrate | Artemia salina | |
Mn2+ | 86% of the activity with Mg2+ in P1,P4-bis(5'-guanosyl)tetraphosphate synthesis | Artemia salina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
490000 | - |
gel filtration | Artemia salina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + GTP | Artemia salina | enzyme catalyzes synthesis of P1,P4-bis(5'-guanosyl)tetraphosphate during oogenesis in Artemia | diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemia salina | - |
brine shrimp | - |
Purification (Comment) | Organism |
---|---|
partial | Artemia salina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | encysted, yolk platelets of | Artemia salina | - |
platelet | of yolk | Artemia salina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Artemia salina |
0.003 | 0.0066 | partially purified enzyme | Artemia salina |
Storage Stability | Organism |
---|---|
0°C or -15°C, 50% loss of activity after 1 week in buffered 500 mM NaCl | Artemia salina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + GDP | rate of the reaction is low compared with synthesis of P1,P4-bis(5'-guanosyl)tetraphosphate and dependent on other small molecular weight components of yolk platelets | Artemia salina | P1,P3-bis(5'-guanosyl)triphosphate + diphosphate | i.e. GP3G | r | |
GTP + GTP | - |
Artemia salina | P1,P4-bis(5'-guanosyl)tetraphosphate + diphosphate | i.e. GP4G | r | |
GTP + GTP | enzyme catalyzes synthesis of P1,P4-bis(5'-guanosyl)tetraphosphate during oogenesis in Artemia | Artemia salina | diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
P1,P3-bis(5'-guanosyl)triphosphate synthesis, partially purified enzyme, Sephadex G-25 fraction | Artemia salina |
40 | 42 | P1,P4-bis(5'-guanosyl)tetraphosphate synthesis | Artemia salina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6 | - |
Artemia salina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | no activity below and above | Artemia salina |