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Literature summary for 2.7.7.44 extracted from

  • Litterer, L.A.; Plaisance, K.L.; Schnurr, J.A.; Storey, K.K.; Jung, H.J.G.; Gronwald, J.W.; Somers, D.A.
    Biosynthesis of UDP-glucuronic acid in developing soybean embryos: Possible role of UDP-sugar pyrophosphorylase (2006), Physiol. Plant., 128, 200-211.
    View publication on PubMed
Search for more literature for 2.7.7.44

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 1-phospho-alpha-D-glucuronate
-
 Glycine max
0.23
-
 alpha-D-glucose 1-phosphate
-
 Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max Q09WE7 Soybean (L.) Merrill
-

Purification (Commentary)

Purification (Comment) Organism
His-Link affinity resin Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 UDP-GlcA PPase activity increases 3.9fold during embryo development (25-45 daf), the activity increases in proportion to the increase in seed fresh weight during embryo development Glycine max
0.0019
-
 substrate alpha-D-mannose 1-phosphate Glycine max
0.0022
-
 substrate D-glucose 6-phosphate Glycine max
0.0034
-
 substrate N-acetyl-alpha-D-glucosamine 1-phosphate Glycine max
0.097
-
 substrate alpha-D-glucoronic acid 1-phosphate Glycine max
0.11
-
 substrate alpha-D-glucose 1-phosphate Glycine max
0.112
-
 substrate alpha-D-galactose 1-phosphate Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + 1-phospho-alpha-D-glucuronate
-
 Glycine max diphosphate + UDP-alpha-D-glucuronate
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Glycine max ?
-
 ?

Synonyms

Synonyms Comment Organism
UDP-Glc PPase
-
 Glycine max
UDP-glucuronic acid pyrophosphorylase
-
 Glycine max
UDP-sugar pyrophosphorylase gene (USP1)
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 broad pH optimum Glycine max