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Literature summary for 2.7.7.43 extracted from

  • Bose, S.; Purkait, D.; Joseph, D.; Nayak, V.; Subramanian, R.
    Structural and functional characterization of CMP-N-acetylneuraminate synthetase from Vibrio cholerae (2019), Acta Crystallogr. Sect. D, 75, 564-577 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.43

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 DE3 cells Vibrio cholerae

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, hanging drop vapor diffusion method, using 200 mM calcium acetate, 0.1 M imidazole pH 8.0, 10% (w/v) PEG 8000 Vibrio cholerae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 CTP at pH 7.5 and 25°C Vibrio cholerae
0.127
-
 N-acylneuraminate at pH 7.5 and 25°C Vibrio cholerae
1.483
-
 N-glycolneuraminate at pH 7.5 and 25°C Vibrio cholerae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Vibrio cholerae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + N-acylneuraminate Vibrio cholerae
-
 diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-glycolneuraminate Vibrio cholerae N-glycolneuraminate is a relatively weaker substrate than N-acylneuraminate diphosphate + CMP-N-glycolneuraminate
-
 ?
CTP + sialic acid Vibrio cholerae
-
 CMP-sialic acid + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography and gel filtration Vibrio cholerae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
 Vibrio cholerae diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-glycolneuraminate N-glycolneuraminate is a relatively weaker substrate than N-acylneuraminate Vibrio cholerae diphosphate + CMP-N-glycolneuraminate
-
 ?
CTP + sialic acid
-
 Vibrio cholerae CMP-sialic acid + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Vibrio cholerae

Synonyms

Synonyms Comment Organism
CMAS
-
 Vibrio cholerae
CMP-N-acetylneuraminate synthetase
-
 Vibrio cholerae
CMP-sialic acid synthetase
-
 Vibrio cholerae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.21
-
 N-glycolneuraminate at pH 7.5 and 25°C Vibrio cholerae
3.97
-
 CTP at pH 7.5 and 25°C Vibrio cholerae
4.46
-
 N-acylneuraminate at pH 7.5 and 25°C Vibrio cholerae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 N-glycolneuraminate at pH 7.5 and 25°C Vibrio cholerae
55.75
-
 N-acylneuraminate at pH 7.5 and 25°C Vibrio cholerae