Cloned (Comment) | Organism |
---|---|
the N-terminal domain, residues 39-267, and the C-terminal domain, residues 267-432, of CMP-sialic acid synthetase, and CMP-sialic acid synthetase, residues 39-432, are cloned into a pET22b-Strep vector | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
the structure of the C-terminal domain of CMP-sialic acid synthetase is solved to 1.9 A resolution | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
CTP | CMP-sialic acid synthetase | Mus musculus | |
0.042 | - |
CTP | N-terminal domain of CMP-sialic acid synthetase | Mus musculus | |
0.043 | - |
CTP | N-terminal domain and C-terminal domain of CMP-sialic acid synthetase | Mus musculus | |
0.045 | - |
sialic acid | CMP-sialic acid synthetase, substrate N-acetylneuraminic acid | Mus musculus | |
0.063 | - |
sialic acid | N-terminal domain and C-terminal domain of CMP-sialic acid synthetase, substrate N-acetylneuraminic acid | Mus musculus | |
0.07 | - |
sialic acid | N-terminal domain of CMP-sialic acid synthetase, substrate N-acetylneuraminic acid | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18300 | - |
C-terminal domain of CMP-sialic acid synthetase, calculated | Mus musculus |
26400 | - |
N-terminal domain of CMP-sialic acid synthetase, calculated | Mus musculus |
44400 | - |
CMP-sialic acid synthetase, calculated | Mus musculus |
56600 | - |
dimer, N-terminal domain of CMP-sialic acid synthetase, determined by gel filtration | Mus musculus |
87000 | - |
tetramer, C-terminal domain of CMP-sialic acid synthetase, determined by gel filtration | Mus musculus |
175800 | - |
tetramer, CMP-sialic acid synthetase, determined by gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + sialic acid | Mus musculus | - |
CMP-sialic acid + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q99KK2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins are expressed in Escherichia coli BL21DE3 cells and purified by affinity chromatography utilizing the StrepII-Tag, further purified on a Superdex 200 HR 10/30 columnn | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + sialic acid | - |
Mus musculus | CMP-sialic acid + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | dimer of dimers | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
CMP-sialic acid synthetase | - |
Mus musculus |
CSS | - |
Mus musculus |
cytidine monophosphate-sialic acid synthetase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
activity assay | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.29 | - |
sialic acid | CMP-sialic acid synthetase, substrate N-acetylneuraminic acid | Mus musculus | |
2.57 | - |
CTP | CMP-sialic acid synthetase | Mus musculus | |
3.47 | - |
sialic acid | N-terminal domain and C-terminal domain of CMP-sialic acid synthetase, substrate N-acetylneuraminic acid | Mus musculus | |
3.56 | - |
sialic acid | N-terminal domain of CMP-sialic acid synthetase, substrate N-acetylneuraminic acid | Mus musculus | |
3.87 | - |
CTP | N-terminal domain and C-terminal domain of CMP-sialic acid synthetase | Mus musculus | |
3.89 | - |
CTP | N-terminal domain of CMP-sialic acid synthetase | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Mus musculus |