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Literature summary for 2.7.7.43 extracted from
- Characterization of a bifunctional cytidine 5-monophosphate N-acetylneuraminic acid synthetase cloned from Streptococcus agalactiae (2006), Biotechnol. Lett., 28, 107-113.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | DTT has no effect on enzyme activity | Streptococcus agalactiae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli as a His6-tagged fusion protein | Streptococcus agalactiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | amino acid sequence alignment of SaV CSS indicate that the C-terminus belongs to the newly discovered SGNH-hydrolase subfamily of serine esterases/lipases | Streptococcus agalactiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | enzyme is inactive in the presence of 5 mM EDTA | Streptococcus agalactiae |  |
| Mn2+ | both catalytic functions are impaired by high concentrations of Mn2+ | Streptococcus agalactiae | |
| additional information | no activity is detected when the enzyme is substituted with 5-35 mM Co2+ or Ca2+ | Streptococcus agalactiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
N-glycolylneuraminic acid | - |
Streptococcus agalactiae | |
| 0.62 | - |
deaminoneuraminic acid | - |
Streptococcus agalactiae | |
| 0.96 | - |
CTP | - |
Streptococcus agalactiae | |
| 5.6 | - |
Neu5Ac | - |
Streptococcus agalactiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for CMP-sialic acid synthetase activity but not for acetylhydrolase activity | Streptococcus agalactiae | |
| Mn2+ | required for CMP-sialic acid synthetase activity but not for acetylhydrolase activity, maximum activity with 4 mM MnCl | Streptococcus agalactiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus agalactiae | Q9AFG9 | serotype V strain2603 V/R | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| C-His6-tagged CSS is purified using a nickel-nitrilotriacetic acid-agarose (Ni2+-NTA-agarose) affinity column | Streptococcus agalactiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deaminoneuraminic acid + CTP | N-glycolylneuraminic acid (Neu5Gc) and deaminoneuraminic acid (KDN) are also substrates | Streptococcus agalactiae | CMP-KDN + diphosphate | - |
? | |
| additional information | SaV CSS is a bifunctional enzyme having both CMP-sialic acid synthetase and acetylhydrolase (acylesterase) activities | Streptococcus agalactiae | ? | - |
? | |
| N-acetylneuraminic acid + CTP | N-glycolylneuraminic acid (Neu5Gc) and deaminoneuraminic acid (KDN) are also substrates | Streptococcus agalactiae | CMP-Neu5Ac + diphosphate | - |
? | |
| N-glycolylneuraminic acid + CTP | N-glycolylneuraminic acid (Neu5Gc) and deaminoneuraminic acid (KDN) are also substrates | Streptococcus agalactiae | CMP-Neu5Gc + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMP-sialic acid synthetase (CSS) | - |
Streptococcus agalactiae |
| cytidine 5-monophosphate N-acetylneuraminic acid synthetase | - |
Streptococcus agalactiae |
| SaV CSS | - |
Streptococcus agalactiae |
| sialic acid cytidylyltransferase | - |
Streptococcus agalactiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptococcus agalactiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme is more stable at lower temperatures, 55% of enzyme activity remains after 24 h incubation at 25°C | Streptococcus agalactiae |
| 37 | - |
enzyme is not stable at 37°C, incubation for 1 h decreases enzyme activity by 50%, incubation for 24 h at 37°C completely inactivates enzyme activity | Streptococcus agalactiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
deaminoneuraminic acid | - |
Streptococcus agalactiae | |
| 0.68 | - |
N-glycolylneuraminic acid | - |
Streptococcus agalactiae | |
| 14 | - |
CTP | - |
Streptococcus agalactiae | |
| 16 | - |
Neu5Ac | - |
Streptococcus agalactiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
optimal CMP-sialic acid synthetase activity | Streptococcus agalactiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.5 | enzyme is active in a wide pH-range | Streptococcus agalactiae |
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