Cloned (Comment) | Organism |
---|---|
the gene is cloned into a T7 expression vector, the protein is expressed in Escherichia coli | [Haemophilus] ducreyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
CTP | pH 7.1, 37°C | [Haemophilus] ducreyi | |
0.26 | - |
N-acetylneuraminate | pH 7.1, 37°C | [Haemophilus] ducreyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, maximal activity at 20 mM | [Haemophilus] ducreyi | |
Mn2+ | 20 mM, 40% of the activation with Mg2+ | [Haemophilus] ducreyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
47000-74000 Da, gel filtration | [Haemophilus] ducreyi |
25440 | - |
2 * 25440, dimer or larger oligomer, electrospray mass spectrometry | [Haemophilus] ducreyi |
25600 | - |
2 * 25600, dimer or larger oligomer, SDS-PAGE | [Haemophilus] ducreyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | [Haemophilus] ducreyi | the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | [Haemophilus] ducreyi 35000 | the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase | diphosphate + CMP-N-acylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Haemophilus] ducreyi | - |
- |
- |
[Haemophilus] ducreyi 35000 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Haemophilus] ducreyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.66 | - |
- |
[Haemophilus] ducreyi |
Storage Stability | Organism |
---|---|
-20°C or -80°C, the relatively crude preparation is stable to storage as a 50% glycerol solution for at least several months | [Haemophilus] ducreyi |
4°C, the relatively crude preparation is stable to storage as a 65% ammonium sulfate suspension or 20% glycerol solution for at least several months | [Haemophilus] ducreyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
[Haemophilus] ducreyi | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase | [Haemophilus] ducreyi | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
[Haemophilus] ducreyi 35000 | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase | [Haemophilus] ducreyi 35000 | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-glycolylneuraminate | - |
[Haemophilus] ducreyi | diphosphate + CMP-N-glycolylneuraminate | - |
? | |
CTP + N-glycolylneuraminate | - |
[Haemophilus] ducreyi 35000 | diphosphate + CMP-N-glycolylneuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25440, dimer or larger oligomer, electrospray mass spectrometry | [Haemophilus] ducreyi |
dimer | 2 * 25600, dimer or larger oligomer, SDS-PAGE | [Haemophilus] ducreyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
[Haemophilus] ducreyi |