Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.43 extracted from

  • Tullius, M.V.; Munson, R.S., Jr.; Wang, J.; Gibson, B.W.
    Purification, cloning, and expression of a cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from Haemophilus ducreyi (1996), J. Biol. Chem., 271, 15373-15380.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the gene is cloned into a T7 expression vector, the protein is expressed in Escherichia coli [Haemophilus] ducreyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
CTP pH 7.1, 37°C [Haemophilus] ducreyi
0.26
-
N-acetylneuraminate pH 7.1, 37°C [Haemophilus] ducreyi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, maximal activity at 20 mM [Haemophilus] ducreyi
Mn2+ 20 mM, 40% of the activation with Mg2+ [Haemophilus] ducreyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
47000-74000 Da, gel filtration [Haemophilus] ducreyi
25440
-
2 * 25440, dimer or larger oligomer, electrospray mass spectrometry [Haemophilus] ducreyi
25600
-
2 * 25600, dimer or larger oligomer, SDS-PAGE [Haemophilus] ducreyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + N-acylneuraminate [Haemophilus] ducreyi the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate [Haemophilus] ducreyi 35000 the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase diphosphate + CMP-N-acylneuraminate
-
?

Organism

Organism UniProt Comment Textmining
[Haemophilus] ducreyi
-
-
-
[Haemophilus] ducreyi 35000
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Haemophilus] ducreyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.66
-
-
[Haemophilus] ducreyi

Storage Stability

Storage Stability Organism
-20°C or -80°C, the relatively crude preparation is stable to storage as a 50% glycerol solution for at least several months [Haemophilus] ducreyi
4°C, the relatively crude preparation is stable to storage as a 65% ammonium sulfate suspension or 20% glycerol solution for at least several months [Haemophilus] ducreyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
[Haemophilus] ducreyi diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase [Haemophilus] ducreyi diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
[Haemophilus] ducreyi 35000 diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate the enzyme produces CMP-N-acetylneuraminate, the nucleotide sugar donor used by sialyltransferase [Haemophilus] ducreyi 35000 diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-glycolylneuraminate
-
[Haemophilus] ducreyi diphosphate + CMP-N-glycolylneuraminate
-
?
CTP + N-glycolylneuraminate
-
[Haemophilus] ducreyi 35000 diphosphate + CMP-N-glycolylneuraminate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 25440, dimer or larger oligomer, electrospray mass spectrometry [Haemophilus] ducreyi
dimer 2 * 25600, dimer or larger oligomer, SDS-PAGE [Haemophilus] ducreyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
[Haemophilus] ducreyi