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Literature summary for 2.7.7.43 extracted from

  • Haft, R.F.; Wessels, M.R.
    Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci (1994), J. Bacteriol., 176, 7372-7374.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates but enzyme is not dependent on Streptococcus sp.

General Stability

General Stability Organism
the enzyme tolerates flash freezing and lyophilization Streptococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
CTP
-
Streptococcus sp.
7.6
-
N-acetylneuraminate
-
Streptococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Streptococcus sp.
Mg2+ required Streptococcus sp.
Mg2+ maximal activity at 20 mM or above Streptococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + N-acylneuraminate Streptococcus sp. the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate Streptococcus sp. Ib the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide diphosphate + CMP-N-acylneuraminate
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus sp.
-
group B, high-producing type Ib strain
-
Streptococcus sp. Ib
-
group B, high-producing type Ib strain
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.354
-
-
Streptococcus sp.

Storage Stability

Storage Stability Organism
-80°C, completely stable for 8 months Streptococcus sp.
4°C, pH 7.6, 83% of the original activity is recovered after 4 weeks Streptococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + N-acylneuraminate
-
Streptococcus sp. diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide Streptococcus sp. diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate
-
Streptococcus sp. Ib diphosphate + CMP-N-acylneuraminate
-
?
CTP + N-acylneuraminate the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide Streptococcus sp. Ib diphosphate + CMP-N-acylneuraminate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37
-
Streptococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 42 4°C: activity is undetectable, 25-37°C: maximal activity, 42°C: 12% of maximal activity Streptococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3 9.4
-
Streptococcus sp.