Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates but enzyme is not dependent on | Streptococcus sp. |
General Stability | Organism |
---|---|
the enzyme tolerates flash freezing and lyophilization | Streptococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
CTP | - |
Streptococcus sp. | |
7.6 | - |
N-acetylneuraminate | - |
Streptococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no effect | Streptococcus sp. | |
Mg2+ | required | Streptococcus sp. | |
Mg2+ | maximal activity at 20 mM or above | Streptococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | Streptococcus sp. | the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | Streptococcus sp. Ib | the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide | diphosphate + CMP-N-acylneuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sp. | - |
group B, high-producing type Ib strain | - |
Streptococcus sp. Ib | - |
group B, high-producing type Ib strain | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.354 | - |
- |
Streptococcus sp. |
Storage Stability | Organism |
---|---|
-80°C, completely stable for 8 months | Streptococcus sp. |
4°C, pH 7.6, 83% of the original activity is recovered after 4 weeks | Streptococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + N-acylneuraminate | - |
Streptococcus sp. | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide | Streptococcus sp. | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | - |
Streptococcus sp. Ib | diphosphate + CMP-N-acylneuraminate | - |
? | |
CTP + N-acylneuraminate | the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide | Streptococcus sp. Ib | diphosphate + CMP-N-acylneuraminate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | - |
Streptococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 42 | 4°C: activity is undetectable, 25-37°C: maximal activity, 42°C: 12% of maximal activity | Streptococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | 9.4 | - |
Streptococcus sp. |