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Literature summary for 2.7.7.41 extracted from

  • Lilley, A.C.; Major, L.; Young, S.; Stark, M.J.; Smith, T.K.
    The essential roles of cytidine diphosphate-diacylglycerol synthase in bloodstream form Trypanosoma brucei (2014), Mol. Microbiol., 92, 453-470.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Tb927.7.220, DNA and amino acid sequence determination and analysis, phylogenetic tree. The protozoan homologous have N-terminal extensions as well as several insertions, making these gene products significantly larger than the average prokaryotic CDS. TbCDS gene Tb927.7.220 can complement the Saccharomyces cerevisiae cds1::KanMX knockout. Recombinant expression of HA-tagged enzyme in enzyme-deficient cells Trypanosoma brucei brucei

Protein Variants

Protein Variants Comment Organism
additional information generation of a conditional CDS knockout in the bloodstream form parasite, phenotype, overview Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum between the nucleus and the kinetoplast Trypanosoma brucei brucei 5783
-
Golgi apparatus
-
Trypanosoma brucei brucei 5794
-
membrane an integral membrane protein with multiple membrane spanning domains Trypanosoma brucei brucei 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, SDS-PAGE Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + phosphatidate Trypanosoma brucei brucei
-
diphosphate + CDP-diacylglycerol
-
?
CTP + phosphatidate Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927
-
diphosphate + CDP-diacylglycerol
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q57U32
-
-
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 Q57U32
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phosphatidate
-
Trypanosoma brucei brucei diphosphate + CDP-diacylglycerol
-
?
CTP + phosphatidate
-
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 diphosphate + CDP-diacylglycerol
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
CDP-DAG synthase
-
Trypanosoma brucei brucei
CDS
-
Trypanosoma brucei brucei
Tb927.7.220
-
Trypanosoma brucei brucei
TbCDS
-
Trypanosoma brucei brucei

General Information

General Information Comment Organism
evolution Trypanosoma cruzi and Leishmaniasp. have both a eukaryotic and prokaryotic version, but Trypanosoma brucei has likely lost its prokaryotic homologue in the recent past Trypanosoma brucei brucei
malfunction biochemical phenotype of the TbCDS conditional knockout, overvie. Lipid extracts from TbCDS CKO have a similar distribution of molecular species to that of wild-type cells across the phospholipid classes to that of wild-type cells, but an increase in diacylglycerols and triacylglycerols i detected. The decrease in CDS activity causes a marked reduction in phosphatidylinositol levels, the disproportional reduction implies the C36:1 species turns over faster than the C40:4 species. Incorporation of [3H]inositol into phosphatidylinositol and phosphatidylinositol phosphate seems relatively unaffected in the TbCDS CKO, but the mutant shows a significant reduction in flux through the GPI pathway Trypanosoma brucei brucei
metabolism cytidine diphosphate diacylglycerol (CDP-DAG) is a central lipid intermediate for several pathways in both prokaryotes and eukaryotes, being produced by CDP-DAG synthase Trypanosoma brucei brucei
physiological function TbCDS is essential for formation of glycosylphosphatidylinositol intermediates. The enzyme is required for synthesis of CDP-DAG for the synthesis of the bulk of phosphatidylinositol in the cell, it produces CDP-DAG required for the endoplasmic reticulum localized phosphatidylinositol synthase to make phosphatidylinositol for GPI anchors Trypanosoma brucei brucei