Cloned (Comment) | Organism |
---|---|
gene Tb927.7.220, DNA and amino acid sequence determination and analysis, phylogenetic tree. The protozoan homologous have N-terminal extensions as well as several insertions, making these gene products significantly larger than the average prokaryotic CDS. TbCDS gene Tb927.7.220 can complement the Saccharomyces cerevisiae cds1::KanMX knockout. Recombinant expression of HA-tagged enzyme in enzyme-deficient cells | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a conditional CDS knockout in the bloodstream form parasite, phenotype, overview | Trypanosoma brucei brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | between the nucleus and the kinetoplast | Trypanosoma brucei brucei | 5783 | - |
Golgi apparatus | - |
Trypanosoma brucei brucei | 5794 | - |
membrane | an integral membrane protein with multiple membrane spanning domains | Trypanosoma brucei brucei | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | Trypanosoma brucei brucei | - |
diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | - |
diphosphate + CDP-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q57U32 | - |
- |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | Q57U32 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | - |
Trypanosoma brucei brucei | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | - |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | diphosphate + CDP-diacylglycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
CDP-DAG synthase | - |
Trypanosoma brucei brucei |
CDS | - |
Trypanosoma brucei brucei |
Tb927.7.220 | - |
Trypanosoma brucei brucei |
TbCDS | - |
Trypanosoma brucei brucei |
General Information | Comment | Organism |
---|---|---|
evolution | Trypanosoma cruzi and Leishmaniasp. have both a eukaryotic and prokaryotic version, but Trypanosoma brucei has likely lost its prokaryotic homologue in the recent past | Trypanosoma brucei brucei |
malfunction | biochemical phenotype of the TbCDS conditional knockout, overvie. Lipid extracts from TbCDS CKO have a similar distribution of molecular species to that of wild-type cells across the phospholipid classes to that of wild-type cells, but an increase in diacylglycerols and triacylglycerols i detected. The decrease in CDS activity causes a marked reduction in phosphatidylinositol levels, the disproportional reduction implies the C36:1 species turns over faster than the C40:4 species. Incorporation of [3H]inositol into phosphatidylinositol and phosphatidylinositol phosphate seems relatively unaffected in the TbCDS CKO, but the mutant shows a significant reduction in flux through the GPI pathway | Trypanosoma brucei brucei |
metabolism | cytidine diphosphate diacylglycerol (CDP-DAG) is a central lipid intermediate for several pathways in both prokaryotes and eukaryotes, being produced by CDP-DAG synthase | Trypanosoma brucei brucei |
physiological function | TbCDS is essential for formation of glycosylphosphatidylinositol intermediates. The enzyme is required for synthesis of CDP-DAG for the synthesis of the bulk of phosphatidylinositol in the cell, it produces CDP-DAG required for the endoplasmic reticulum localized phosphatidylinositol synthase to make phosphatidylinositol for GPI anchors | Trypanosoma brucei brucei |