Protein Variants | Comment | Organism |
---|---|---|
C1226T/C1227T/P363S | construction of loss-of-function mutation in CDP-DG synthase in Schizosaccharomyces japonicus bbl1 mutant cells, the mutation triggers abnormal lipid droplet biogenesis and triacylglycerol accumulation. The P363S mutation in the Cds1 enzyme leads to a reduction in its CDP-DG synthase activity and potential shunting of a bulk of cellular phosphatidate into triacylglycerol biosynthesis through the DG pathway. The cds1bbl1 mutation is recessive, since introduction of the wild-type gene as an extra copy fully rescues the phenotype of bbl1 cells. Mutant bbl1 phenotype, overview | Schizosaccharomyces japonicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Schizosaccharomyces pombe | 5783 | - |
endoplasmic reticulum | - |
Schizosaccharomyces japonicus | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | Schizosaccharomyces pombe | - |
diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | Schizosaccharomyces japonicus | - |
diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | Schizosaccharomyces pombe 972 | - |
diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | Schizosaccharomyces japonicus yFS275 | - |
diphosphate + CDP-diacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces japonicus | - |
- |
- |
Schizosaccharomyces japonicus | B6JVL2 | - |
- |
Schizosaccharomyces japonicus yFS275 | B6JVL2 | - |
- |
Schizosaccharomyces pombe | Q9P381 | - |
- |
Schizosaccharomyces pombe 972 | Q9P381 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | - |
Schizosaccharomyces pombe | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | - |
Schizosaccharomyces japonicus | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | - |
Schizosaccharomyces pombe 972 | diphosphate + CDP-diacylglycerol | - |
? | |
CTP + phosphatidate | - |
Schizosaccharomyces japonicus yFS275 | diphosphate + CDP-diacylglycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDP-DG synthase | - |
Schizosaccharomyces pombe |
CDP-DG synthase | - |
Schizosaccharomyces japonicus |
CDS1 | - |
Schizosaccharomyces pombe |
CDS1 | - |
Schizosaccharomyces japonicus |
SJAG_00426 | - |
Schizosaccharomyces japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
assay at | Schizosaccharomyces pombe |
36 | - |
assay at | Schizosaccharomyces japonicus |
General Information | Comment | Organism |
---|---|---|
evolution | Cds1 is an evolutionarily conserved protein | Schizosaccharomyces pombe |
evolution | Cds1 is an evolutionarily conserved protein | Schizosaccharomyces japonicus |
malfunction | cycling bbl1 mutant cells deficient in the function of the endoplasmic reticlum-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the endoplasmic reticlum membranes. These unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway. The bbl1 mutant enzyme is catalytically deficient, penotype, overview | Schizosaccharomyces japonicus |
malfunction | cycling cells deficient in the function of the endoplasmic reticlum-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the endoplasmic reticlum membranes. These unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycerol-rich lifestyle reliant on the Kennedy pathway | Schizosaccharomyces pombe |
physiological function | cycling cells deficient in the function of the ER-resident CDP-diacylglycerol synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. These structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion | Schizosaccharomyces japonicus |
physiological function | CDP-DG synthase is responsible for a rate-limiting step in the production of phospholipids. It catalyzes condensation of phosphatidate and cytidine triphosphate, releasing the CDP-diacylglycerol and diphosphate products | Schizosaccharomyces pombe |
physiological function | CDP-DG synthase is responsible for a rate-limiting step in the production of phospholipids. It catalyzes condensation of phosphatidate and cytidine triphosphate, releasing the CDP-diacylglycerol and diphosphate products | Schizosaccharomyces japonicus |