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Literature summary for 2.7.7.41 extracted from
- CDP-diacylglycerol synthase activity in Clostridium perfringens (1982), Appl. Environ. Microbiol., 43, 81-85.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 5 mM, about 9fold stimulation | Clostridium perfringens |  |
General Stability
| General Stability | Organism |
|---|---|
| stable to at least 2 cycles of freezing and thawing | Clostridium perfringens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
CTP | pH 7.0, 30°C | Clostridium perfringens | |
| 0.22 | - |
phosphatidic acid | pH 7.0, 30°C | Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell envelope | associated with cell envelope fraction | Clostridium perfringens | 30313 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required, Mn2+ or Mg2+, maximal activity at 5 mM Mg2+. Maximal activity obtained with Mg2+ is about 1.2fold greater than that obtained with Mn2+ | Clostridium perfringens | |
| Mn2+ | divalent cation required, Mn2+ or Mg2+, maximal activity at 1 mM Mn2+ | Clostridium perfringens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, cell envelope preparations stable for at least 2 months | Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + phosphatidate | - |
Clostridium perfringens | diphosphate + CDPdiacylglycerol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Clostridium perfringens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 35% of maximal activity, 60°C: about 45% of maximal activity | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Clostridium perfringens |
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