General Stability | Organism |
---|---|
the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
phosphatidic acid | - |
Escherichia coli | |
0.58 | - |
dCTP | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Escherichia coli |
27570 | - |
x * 27570, calculation from gene sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | Escherichia coli | the activity is essential for all phospholipid biosynthesis in Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.79 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
purified enzyme can be stored frozen for months if quick-frozen in liquid nitrogen | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | the enzyme is specific for long-chain phosphatidic acid. 1-Acyl-sn-glycero-3-phosphate is not a substrate and phosphatidic acids with acyl chains shorter than 16 carbons are poor substrates | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? | |
CTP + phosphatidate | the activity is essential for all phospholipid biosynthesis in Escherichia coli | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
? | |
dCTP + phosphatidate | - |
Escherichia coli | diphosphate + dCDPdiacylglycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Escherichia coli |
? | x * 27570, calculation from gene sequence | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Escherichia coli |