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Literature summary for 2.7.7.40 extracted from

  • Zolli, M.; Kobric, D.J.; Brown, E.D.
    Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae (2001), Biochemistry, 40, 5041-5048.
    View publication on PubMed
Search for more literature for 2.7.7.40

Cloned(Commentary)

Cloned (Comment) Organism
-
 Haemophilus influenzae

Protein Variants

Protein Variants Comment Organism
K368A kinetic values similar to wild-type enzyme Haemophilus influenzae
R18A reduced enzyme activity Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
 CTP pH 7.2, 25°C, K386A mutant Haemophilus influenzae
0.099
-
 CTP pH 7.2, 25°C Haemophilus influenzae
0.12
-
 D-ribitol 5-phosphate pH 7.2, 25°C, K386A mutant Haemophilus influenzae
0.13
-
 D-ribitol 5-phosphate pH 7.2, 25°C Haemophilus influenzae
0.39
-
 CTP pH 7.2, 25°C, R18A mutant Haemophilus influenzae
1
-
 D-ribitol 5-phosphate pH 7.2, 25°C, R18A mutant Haemophilus influenzae

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + D-ribitol 5-phosphate Haemophilus influenzae
-
 diphosphate + CDPribitol
-
 ?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + D-ribitol 5-phosphate
-
 Haemophilus influenzae diphosphate + CDPribitol
-
 ?
additional information enzyme has also ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae ?
-
 ?
additional information no activity with ATP, GTP, UTP, ADP or CDP Haemophilus influenzae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 D-ribitol 5-phosphate pH 7.2, 25°C, R18A mutant Haemophilus influenzae
9.7
-
 D-ribitol 5-phosphate pH 7.2, 25°C, K386A mutant Haemophilus influenzae
13
-
 D-ribitol 5-phosphate pH 7.2, 25°C, wild-type enzyme Haemophilus influenzae