Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Haemophilus influenzae |
General Stability | Organism |
---|---|
unstable, 50% loss of activity after 2 h at 23°C in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.05 mM CTP | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
D-ribitol 5-phosphate | pH 7.1, 30°C | Haemophilus influenzae | |
0.15 | - |
CTP | pH 7.1, 30°C | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + D-ribitol 5-phosphate | Haemophilus influenzae | - |
diphosphate + CDPribitol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme copurifies with ribitol 5-phosphate dehydrogenase activity | Haemophilus influenzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.7 | - |
CDP-ribitol pyrophosphorylase activity | Haemophilus influenzae |
37.5 | - |
ribitol 5-phosphate dehydrogenase activity | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + D-arabitol 5-phosphate | - |
Haemophilus influenzae | diphosphate + CDParabitol | - |
? | |
CTP + D-ribitol 5-phosphate | - |
Haemophilus influenzae | diphosphate + CDPribitol | - |
? | |
additional information | enzyme has also ribitol 5-phosphate dehydrogenase activity | Haemophilus influenzae | ? | - |
? | |
additional information | no activity with erythritol 4-phosphate, sorbitol 6-phosphate and UTP | Haemophilus influenzae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Haemophilus influenzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
50% loss of activity after 2 h in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.005 mM CTP | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
same enzyme activity at pH 7.1 and pH 8 | Haemophilus influenzae |
8 | - |
same enzyme activity at pH 7.1 and pH 8 | Haemophilus influenzae |