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Literature summary for 2.7.7.40 extracted from

  • Follens, A.; Veiga-da-Cunha, M.; Merckx, R.; van Schaftingen, E.; van Eldere, J.
    acs1 of Haemophilus influenzae type a capsulation locus region II encodes a bifunctional ribulose 5-phosphate reductase- CDP-ribitol pyrophosphorylase (1999), J. Bacteriol., 181, 2001-2007.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Haemophilus influenzae

General Stability

General Stability Organism
unstable, 50% loss of activity after 2 h at 23°C in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.05 mM CTP Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
D-ribitol 5-phosphate pH 7.1, 30°C Haemophilus influenzae
0.15
-
CTP pH 7.1, 30°C Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
SDS-PAGE Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + D-ribitol 5-phosphate Haemophilus influenzae
-
diphosphate + CDPribitol
-
?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme copurifies with ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.7
-
CDP-ribitol pyrophosphorylase activity Haemophilus influenzae
37.5
-
ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + D-arabitol 5-phosphate
-
Haemophilus influenzae diphosphate + CDParabitol
-
?
CTP + D-ribitol 5-phosphate
-
Haemophilus influenzae diphosphate + CDPribitol
-
?
additional information enzyme has also ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae ?
-
?
additional information no activity with erythritol 4-phosphate, sorbitol 6-phosphate and UTP Haemophilus influenzae ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Haemophilus influenzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
23
-
50% loss of activity after 2 h in 20 mM HEPES, 1 mM DTT and 0.5mg/ml bovine serum albumin, decrease of activity can be prevented by addition of 0.005 mM CTP Haemophilus influenzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
same enzyme activity at pH 7.1 and pH 8 Haemophilus influenzae
8
-
same enzyme activity at pH 7.1 and pH 8 Haemophilus influenzae